IP (Immunoprecipitation)
(SNW1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SNW1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SNW1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 61 kDaObserved band size :72 kDa)
Rabbit anti-Human SNW1 Polyclonal Antibody | anti-SNW1 antibody
Anti-SNW1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
SNW1, Antibody; Anti-SNW1 Antibody, Rabbit Polyclonal; SNW1 Antibody, Rabbit PAb, Antigen Affinity Purified; SNW domain containing 1; Anti-Bx42 Antibody; Anti-NCOA-62 Antibody; Anti-Prp45 Antibody; Anti-PRPF45 Antibody; Anti-SKIIP Antibody; Anti-SKIP Antibody; anti-SNW1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SNW1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SNW1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
E Coli-derived Human SNW1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SNW1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SNW1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SNW1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SNW1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 61 kDaObserved band size :72 kDa)
IP (Immunoprecipitation)
(SNW1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SNW1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SNW1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 61 kDaObserved band size :72 kDa)
WB (Western Blot)
(Anti-SNW1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:61 kDaObserved band size:72 kDa)
WB (Western Blot)
(Anti-SNW1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HEK293 Whole Cell LysateLane C: HepG2 Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:61 kDaObserved band size:72 kDa)
Related Product Information for anti-SNW1 antibody
SNW1 is a nuclear receptor co-activator involved in splicing and transcription control, including androgen receptor signaling. Overexpression of SNW1 has been linked to adverse prognosis in different cancer types. SNW1 is a nuclear protein known to regulate gene expression. Through its ability to regulate a specific domain of Bone morphogenetic protein (BMP) activity in the vertebrate embryo, SNW1 is a critical regulator of neural plate border formation and thus neural crest specification. The Ski-interacting protein (SNW1) acts as a transcriptional co-regulator associated with cell cycle progression, acute and chronic inflammatory responses, and its role involves binding to the NF-kappaB-p-TEFb complex to facilitate transcriptional elongation of some NF-kappaB target genes. Prp45, the SNW/SKIP ortholog in yeast, is assumed to be essential for pre-mRNA processing.
Product Categories/Family for anti-SNW1 antibody
References
Zhang Q, et al. (2020) Snw1 interacts with ikkgamma to positively regulate antiviral innate immune responses against influenza a virus infection. Microbes Infect 22 (10): 576-584.Wu MY, et al. (2011) Snw1 is a critical regulator of spatial bmp activity, neural plate border formation, and neural crest specification in vertebrate embryos. PLoS Biol 9 (2): e1000593.Verma S, et al. (2019) Snw1, a novel transcriptional regulator of the nf-kappab pathway. Mol Cell Biol 39 (3): e00415-18.Hoflmayer D, et al. (2019) Snw1 is a prognostic biomarker in prostate cancer. Diagn Pathol 14 (1): 33.
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Product Notes
The SNW1 (Catalog #AAA256081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SNW1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SNW1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SNW1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNW1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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