FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-SOCS3 antibody (AAA125532).Overlay histogram showing SiHa cells stained with AAA125532 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOCS3 Antibody (AAA125532, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit SOCS3 Polyclonal Antibody | anti-SOCS3 antibody
Anti-SOCS3 Antibody
Gene Names
SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
SOCS3, Antibody; Anti-SOCS3 Antibody; ATOD4 antibody; CIS 3 antibody; CIS-3 antibody; CIS3 antibody; Cish3 antibody; Cytokine induced SH2 protein 3 antibody; Cytokine-inducible SH2 protein 3 antibody; E2a Pbx1 target gene in fibroblasts 10 antibody; EF 10 antibody; MGC71791 antibody; SOCS 3 antibody; SOCS-3 antibody; Socs3 antibody; SOCS3_HUMAN antibody; SSI 3 antibody; SSI-3 antibody; SSI3 antibody; STAT induced STAT inhibitor 3 antibody; STAT-induced STAT inhibitor 3 antibody; Suppressor of cytokine signaling 3 antibody; suppressor of cytokine signaling 3; anti-SOCS3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Rabbit IgG polyclonal antibody for SOCS3 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Applicable Applications for anti-SOCS3 antibody
ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E Coli-derived human SOCS3 recombinant protein (Position: S13-L225).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-SOCS3 antibody (AAA125532).Overlay histogram showing SiHa cells stained with AAA125532 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOCS3 Antibody (AAA125532, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of SiHa cells using anti-SOCS3 antibody (AAA125532).Overlay histogram showing SiHa cells stained with AAA125532 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SOCS3 Antibody (AAA125532, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of SOCS3 using anti-SOCS3 antibody (AAA125532).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: rat heart tissue lysatesLane 3: rat skeletal muscle tissue lysatesLane 4: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody (Catalog # AAA125532) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOCS3 at approximately 28-30KD. The expected band size for SOCS3 is at 28-30KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of SOCS3 using anti-SOCS3 antibody (AAA125532).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: rat heart tissue lysatesLane 3: rat skeletal muscle tissue lysatesLane 4: mouse heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody (Catalog # AAA125532) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOCS3 at approximately 28-30KD. The expected band size for SOCS3 is at 28-30KD.)
Related Product Information for anti-SOCS3 antibody
SOCS3 (Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.
References
1. Babon, J. J., McManus, E. J., Yao, S., DeSouza, D. P., Mielke, L. A., Sprigg, N. S., Willson, T. A., Hilton, D. J., Nicola, N. A., Baca, M., Nicholson, S. E., Norton, R. S. The structure of SOCS3 reveals the basis of the extended SH2 domain function and identifies an unstructured insertion that regulates stability. Molec. Cell 22: 205-216, 2006.
2. Chen, Z., Laurence, A., Kanno, Y., Pacher-Zavisin, M., Zhu, B. -M., Tato, C., Yoshimura, A., Hennighausen, L., O'Shea, J. J. Selective regulatory function of Socs3 in the formation of IL-17-secreting T cells. Proc. Nat. Acad. Sci. 103: 8137-8142, 2006.
3. Jo, D., Liu, D., Yao, S., Collins, R. D., Hawiger, J. Intracellular protein therapy with SOCS3 inhibits inflammation and apoptosis. Nature Med. 11: 892-898, 2005.
2. Chen, Z., Laurence, A., Kanno, Y., Pacher-Zavisin, M., Zhu, B. -M., Tato, C., Yoshimura, A., Hennighausen, L., O'Shea, J. J. Selective regulatory function of Socs3 in the formation of IL-17-secreting T cells. Proc. Nat. Acad. Sci. 103: 8137-8142, 2006.
3. Jo, D., Liu, D., Yao, S., Collins, R. D., Hawiger, J. Intracellular protein therapy with SOCS3 inhibits inflammation and apoptosis. Nature Med. 11: 892-898, 2005.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,770 Da
NCBI Official Full Name
suppressor of cytokine signaling 3
NCBI Official Synonym Full Names
suppressor of cytokine signaling 3
NCBI Official Symbol
SOCS3
NCBI Official Synonym Symbols
CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
NCBI Protein Information
suppressor of cytokine signaling 3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3
UniProt Protein Name
Suppressor of cytokine signaling 3
UniProt Gene Name
SOCS3
UniProt Synonym Gene Names
CIS3; SSI3; SOCS-3; CIS-3; SSI-3
UniProt Entry Name
SOCS3_HUMAN
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Product Notes
The SOCS3 socs3 (Catalog #AAA125532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SOCS3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SOCS3 can be used in a range of immunoassay formats including, but not limited to, ELISA (Direct ELISA), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the SOCS3 socs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOCS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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