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WB (Western Blot) (Anti-SOST Antibody (N-term)at 1:2000 dilution + human kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit SOST Polyclonal Antibody | anti-SOST antibody

SOST Antibody (N-term)

Gene Names
SOST; CDD; VBCH; SOST1
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
SOST; Polyclonal Antibody; SOST Antibody (N-term); Sclerostin; anti-SOST antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SOST.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
12-42
Sequence Length
213
Applicable Applications for anti-SOST antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Anti-SOST Antibody (N-term)at 1:2000 dilution + human kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-SOST Antibody (N-term)at 1:2000 dilution + human kidney lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-SOST Antibody (N-term) at 1:2000 dilutionLane 1: mouse lung lysatesLane 2: human liver lysatesLane 3: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-SOST Antibody (N-term) at 1:2000 dilutionLane 1: mouse lung lysatesLane 2: human liver lysatesLane 3: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-SOST Antibody (N-term)at 1:2000 dilution + mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-SOST Antibody (N-term)at 1:2000 dilution + mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-SOST Antibody (N-term)at 1:2000 dilution + human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-SOST Antibody (N-term)at 1:2000 dilution + human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-SOST Antibody (N-term) at 1:1000 dilutionLane 1: human kidney lysatesLane 2: human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-SOST Antibody (N-term) at 1:1000 dilutionLane 1: human kidney lysatesLane 2: human liver lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(All lanes : Anti-SOST Antibody (N-term) at 1:2000 dilutionLane 1: mouse lung lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (All lanes : Anti-SOST Antibody (N-term) at 1:2000 dilutionLane 1: mouse lung lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-SOST Antibody (N-term)at 1:2000 dilution + mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot) (Anti-SOST Antibody (N-term)at 1:2000 dilution + mouse lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 24 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-SOST antibody
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
Product Categories/Family for anti-SOST antibody
References
Semenov,M.V., J. Biol. Chem. 281 (50), 38276-38284 (2006)
Ellies,D.L., J. Bone Miner. Res. 21 (11), 1738-1749 (2006)
Balemans,W., J Musculoskelet Neuronal Interact 6 (4), 355-356 (2006)
Gardner,J.C., J. Clin. Endocrinol. Metab. 90 (12), 6392-6395 (2005)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24031
NCBI Official Full Name
sclerostin
NCBI Official Synonym Full Names
sclerostin
NCBI Official Symbol
SOST
NCBI Official Synonym Symbols
CDD; VBCH; SOST1
NCBI Protein Information
sclerostin
UniProt Protein Name
Sclerostin
UniProt Gene Name
SOST
UniProt Entry Name
SOST_HUMAN

Similar Products

Product Notes

The SOST sost (Catalog #AAA28704) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 12-42. The SOST Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's SOST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the SOST sost for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SOST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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