IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with SP140 Antibody (Center) (AAA289315). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SP140 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Rabbit anti-Human SP140 Polyclonal Antibody | anti-SP140 antibody
SP140 Antibody (Center)
Gene Names
SP140; LYSP100; LYSP100-A; LYSP100-B
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
SP140, Antibody; SP140 Antibody (Center); Nuclear body protein SP140 {ECO:0000312|HGNC:HGNC:17133}; Lymphoid-restricted homolog of Sp100; LYSp100; Nuclear autoantigen Sp-140; Speckled 140 kDa; SP140 HGNC:17133); anti-SP140 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
This SP140 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the Central region of human SP140.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
272-301
Sequence Length
172
Applicable Applications for anti-SP140 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body (PubMed:8910577). May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection (PubMed:8910577). May play a role in chromatin-mediated regulation of gene expression although it does not bind to histone H3 tails (PubMed:24267382).
Cellular Location
Nucleus. Nucleus, PML body Cytoplasm. Note=Localized to nuclear structures termed LANDS, for LYSp100-associated nuclear domains. LANDS are globular, electron-dense structures most often found in the nucleoplasm, but also found at the nuclear membrane and in the cytoplasm, suggesting that these structures may traffic between the cytoplasm and the nucleus (PubMed:8695863). Also colocalizes with PML in a subset of PML nuclear bodies (PubMed:8910577)
Tissue Location
High levels in spleen and peripheral blood leukocytes, much lower levels in tonsils, thymus, prostate, ovary, small intestine, and colon (PubMed:8695863, PubMed:8910577). Very low levels in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas (PubMed:8910577). Not detected in brain, liver and muscle (PubMed:8695863).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with SP140 Antibody (Center) (AAA289315). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SP140 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with SP140 Antibody (Center) (AAA289315). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SP140 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SP140 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
IHC (Immunohiostchemistry)
(SP140 Antibody (Center) (AAA289315) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP140 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(SP140 Antibody (Center) (AAA289315) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP140 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(SP140 Antibody (Center) (AAA289315) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SP140 antibody detected the SP140 protein (arrow).)
WB (Western Blot)
(SP140 Antibody (Center) (AAA289315) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the SP140 antibody detected the SP140 protein (arrow).)
Related Product Information for anti-SP140 antibody
Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection.
Product Categories/Family for anti-SP140 antibody
References
Slager, S.L., et al. Cancer Epidemiol. Biomarkers Prev. 19(4):1098-1102(2010)
Di Bernardo, M.C., et al. Nat. Genet. 40(10):1204-1210(2008)
Madani, N., et al. J. Virol. 76(21):11133-11138(2002)
Bloch, D.B., et al. J. Biol. Chem. 271(46):29198-29204(1996)
Dent, A.L., et al. Blood 88(4):1423-1426(1996)
Di Bernardo, M.C., et al. Nat. Genet. 40(10):1204-1210(2008)
Madani, N., et al. J. Virol. 76(21):11133-11138(2002)
Bloch, D.B., et al. J. Biol. Chem. 271(46):29198-29204(1996)
Dent, A.L., et al. Blood 88(4):1423-1426(1996)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 98223
NCBI Official Full Name
nuclear body protein SP140 isoform 2
NCBI Official Synonym Full Names
SP140 nuclear body protein
NCBI Official Symbol
SP140
NCBI Official Synonym Symbols
LYSP100; LYSP100-A; LYSP100-B
NCBI Protein Information
nuclear body protein SP140
UniProt Protein Name
Nuclear body protein SP140
UniProt Gene Name
SP140
UniProt Synonym Gene Names
LYSp100
UniProt Entry Name
SP140_HUMAN
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Product Notes
The SP140 sp140 (Catalog #AAA289315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 272-301. The SP140 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SP140 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the SP140 sp140 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SP140, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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