WB (Western Blot)
(Detection of human and mouse SPG8/Strumpellin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPG8/Strumpellin antibody AAA212951 (lot AAA212951-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse SPG8/Strumpellin Polyclonal Antibody | anti-SPG8 antibody
Rabbit anti-SPG8/Strumpellin Antibody, Affinity Purified
Gene Names
KIAA0196; RTSC; SPG8
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SPG8/Strumpellin, Antibody; Rabbit anti-SPG8/Strumpellin Antibody, Affinity Purified; KIAA0196; WASH complex subunit 5; strumpellin; SPG8; RTSC; WASH complex subunit strumpellin; RTSC1; anti-SPG8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-SPG8 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Zebrafish, Orangutan
Immunogen
Between 1109 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse SPG8/Strumpellin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPG8/Strumpellin antibody AAA212951 (lot AAA212951-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse SPG8/Strumpellin by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPG8/Strumpellin antibody AAA212951 (lot AAA212951-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SPG8/Strumpellin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPG8/Strumpellin antibody AAA212951 (lot AAA212951-1) used for IP at 6 ug per reaction. SPG8/Strumpellin was inefficiently immunoprecipitated by rabbit anti-SPG8/Strumpellin antibody BL18606. For blotting immunoprecipitated SPG8/Strumpellin, AAA212951 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human SPG8/Strumpellin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPG8/Strumpellin antibody AAA212951 (lot AAA212951-1) used for IP at 6 ug per reaction. SPG8/Strumpellin was inefficiently immunoprecipitated by rabbit anti-SPG8/Strumpellin antibody BL18606. For blotting immunoprecipitated SPG8/Strumpellin, AAA212951 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-SPG8 antibody
SPG8/Strumpellin acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093). SPG8/Strumpellin may be involved in axonal outgrowth [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q12768].
Product Categories/Family for anti-SPG8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
134,286 Da
NCBI Official Full Name
WASH complex subunit strumpellin
NCBI Official Synonym Full Names
KIAA0196
NCBI Official Symbol
KIAA0196
NCBI Official Synonym Symbols
RTSC; SPG8
NCBI Protein Information
WASH complex subunit strumpellin
UniProt Protein Name
WASH complex subunit strumpellin
UniProt Gene Name
KIAA0196
UniProt Entry Name
STRUM_HUMAN
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Product Notes
The SPG8 kiaa0196 (Catalog #AAA212951) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SPG8/Strumpellin Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPG8/Strumpellin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SPG8 kiaa0196 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPG8/Strumpellin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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