IF (Immunofluorescence)
(Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 μg/mL.)
Rabbit SPP1 Polyclonal Antibody | anti-SPP1 antibody
SPP1 Antibody
Gene Names
SPP1; OPN; BNSP; BSPI; ETA-1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
Purity
SPP1 Antibody is Protein A purified.
Synonyms
SPP1, Antibody; SPP1 Antibody; SPP1; OPN; BNSP; BSPI; ETA-1; PSEC0156; Osteopontin; Bone sialoprotein 1; SPP-1; secreted phosphoprotein 1; anti-SPP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SPP1 antibody is human, mouse and rat reactive. Multiple isoforms of SPP1 are known to exist.
Purity/Purification
SPP1 Antibody is Protein A purified.
Form/Format
Liquid; SPP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
327
Applicable Applications for anti-SPP1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
SPP1 antibody was raised against an 18 amino acid peptide near the amino terminus of human SPP1.
The immunogen is located within amino acids 50-100 of SPP1
The immunogen is located within amino acids 50-100 of SPP1
Homology
Predicted species reactivity based on immunogen sequence: Pig: (94%), Bovine: (83%)
Positive Control
1) Cat. No. ( ) Human Bladder Tissue Lysate.
Preparation and Storage
SPP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
IF (Immunofluorescence)
(Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of SPP1 in human bladder tissue with SPP1 antibody at 20 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of SPP1 in human bladder tissue with SPP1 antibody at 5 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of SPP1 in human bladder tissue with SPP1 antibody at 5 μg/mL.)
WB (Western Blot)
(SPP1 Antibody)
WB (Western Blot)
(SPP1 Antibody)
Related Product Information for anti-SPP1 antibody
SPP1 Antibody: The secreted protein 1 (SPP1), also known as osteopontin, is a major noncollagenous protein of bone, but is also found in the extracellular matrix of other mineralized tissues and in bodily fluids. In bone, SPP1 is produced by osteoblasts, osteocytes, macrophages, and osteoclasts (1,2). SPP1 binds to cells through integrin and non-integrin receptors, as well as the adhesion receptor CD44 in an RGD-independent manner, enabling SPP1 to induce a number of functional effects including macrophage chemotaxis, cytoprotection, and regulation of T helper type 1 cells (2). SPP1 can regulate biomineralization by inhibiting the formation of hydroxyapatite (3) and the growth of calcium oxalate crystals (4).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 36 kDa
Observed: 37 kDa
Observed: 37 kDa
NCBI Official Full Name
osteopontin isoform 5
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
SPP1
NCBI Official Synonym Symbols
OPN; BNSP; BSPI; ETA-1
NCBI Protein Information
osteopontin; uropontin; nephropontin; SPP1/CALPHA1 fusion; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
UniProt Protein Name
Osteopontin
UniProt Gene Name
SPP1
UniProt Synonym Gene Names
BNSP; OPN; SPP-1
UniProt Entry Name
OSTP_HUMAN
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Product Notes
The SPP1 spp1 (Catalog #AAA40751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SPP1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the SPP1 spp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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