Rabbit anti-Human SPRR2A Polyclonal Antibody | anti-SPRR2A antibody

SPRR2A Antibody (C-term)

Cat. Num. AAA28741

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: SPRR2A, Antibody; SPRR2A Antibody (C-term); Small proline-rich protein 2A; SPR-2A; 2-1; SPRR2A; anti-SPRR2A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This SPRR2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-72 amino acids from the C-terminal region of human SPRR2A.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 48-72
• Sequence Length: 72

• Applicable Applications for anti-SPRR2A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:1000
• Function: Gut bactericidal protein that selectively kills Gram-positive bacteria by binding to negatively charged lipids on bacterial membranes, leading to bacterial membrane permeabilization and disruption (PubMed:34735226). Specifically binds lipids bearing negatively charged headgroups, such as phosphatidic acid, phosphatidylserine (PS), cardiolipin (CL), and phosphatidylinositol phosphates, but not to zwitterionic or neutral lipids (PubMed:34735226). Induced by type-2 cytokines in response to helminth infection and is required to protect against helminth-induced bacterial invasion of intestinal tissue (By similarity). May also be involved in the development of the cornified envelope of squamous epithelia; however, additional evidences are required to confirm this result in vivo (PubMed:8325635).
• Cellular Location: Secreted. Secreted, extracellular space. Cytoplasmic vesicle, secretory vesicle. Note=Present in intestinal secretory epithelial cells and is secreted into the intestinal lumen.
• Tissue Location: Expressed in intestine; selectively expressed in goblet cells.
• Preparation and Storage: Maintain refrigerated at 2-8° C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of lysate from A431 cell line, using SPRR2A Antibody (C-term). AAA28741 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

Related Product Information for anti-SPRR2A antibody

Cross-linked envelope protein of keratinocytes. It is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. All that results in the formation of an insoluble envelope beneath the plasma membrane.

Product Categories/Family for anti-SPRR2A antibody

Cell Biology; Signal transduction

References

Kainu, K., et al. Exp. Dermatol. 18(2):109-115(2009)
Demetris, A.J., et al. J. Hepatol. 48(2):276-288(2008)
Tong, L., et al. Invest. Ophthalmol. Vis. Sci. 47(5):1938-1946(2006)
Hong, S.H., et al. Mol. Cells 17(3):477-484(2004)
Cabral, A., et al. J. Biol. Chem. 278(20):17792-17799(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 5174693
• NCBI GeneID: 6700
• NCBI Accession #: NP_005979.1
• NCBI GenBank Nucleotide #: NM_005988.2
• UniProt Accession #: P35326; Q5T529; B2R4T3; D3DV35
• NCBI Official Full Name: small proline-rich protein 2A
• NCBI Official Synonym Full Names: small proline-rich protein 2A
• NCBI Official Symbol: SPRR2A
• NCBI Protein Information: small proline-rich protein 2A
• UniProt Protein Name: Small proline-rich protein 2A
• UniProt Gene Name: SPRR2A
• UniProt Synonym Gene Names: SPR-2A
• UniProt Entry Name: SPR2A_HUMAN