WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SPTBN1 antibody (AAA37541) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit anti-Human, Mouse SPTBN1 Polyclonal Antibody | anti-SPTBN1 antibody
SPTBN1
Gene Names
SPTBN1; ELF; SPTB2; betaSpII
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity purification
Synonyms
SPTBN1, Antibody; SPTBN1; ELF; HEL102; SPTB2; betaSpII; spectrinbeta, non-erythrocytic 1; anti-SPTBN1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
2364
Applicable Applications for anti-SPTBN1 antibody
WB (Western Blot)
Immunogen
Recombinant fusion protein containing a sequencecorresponding to amino acids 2100-2200 of human SPTBN1(NP_003119.2).
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SPTBN1 antibody (AAA37541) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using SPTBN1 antibody (AAA37541) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Related Product Information for anti-SPTBN1 antibody
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasmamembrane to the actin cytoskeleton, and functions in the determination of cell shape,arrangement of transmembrane proteins, and organization of organelles. It iscomposed of two antiparallel dimers of alpha- and beta- subunits. This gene is onemember of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimerformation. Multiple transcript variants encoding different isoforms have been found forthis gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed MW:275kDa
Calculated MW:251kDa/253kDa/274kDa
Calculated MW:251kDa/253kDa/274kDa
NCBI Official Full Name
spectrin beta chain, non-erythrocytic 1 isoform 1
NCBI Official Synonym Full Names
spectrin, beta, non-erythrocytic 1
NCBI Official Symbol
SPTBN1
NCBI Official Synonym Symbols
ELF; SPTB2; betaSpII
NCBI Protein Information
spectrin beta chain, non-erythrocytic 1; beta-fodrin; beta-G spectrin; beta-spectrin 2; beta-II spectrin; beta-spectrin II; fodrin beta chain; embryonic liver beta-fodrin; spectrin beta chain, brain 1; beta-spectrin non-erythrocytic 1; spectrin, non-erythroid beta chain 1
UniProt Protein Name
Spectrin beta chain, non-erythrocytic 1
UniProt Gene Name
SPTBN1
UniProt Synonym Gene Names
SPTB2
UniProt Entry Name
SPTB2_HUMAN
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Product Notes
The SPTBN1 sptbn1 (Catalog #AAA37541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPTBN1 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SPTBN1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SPTBN1 sptbn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SPTBN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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