Rabbit anti-Human SREBF2 Polyclonal Antibody | anti-SREBF2 antibody

SREBF2 Antibody (Center)

Cat. Num. AAA286444

• Gene Names: SREBF2; SREBP2; bHLHd2; SREBP-2
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: SREBF2, Antibody; SREBF2 Antibody (Center); Sterol regulatory element-binding protein 2; SREBP-2; Class D basic helix-loop-helix protein 2; bHLHd2; Sterol regulatory element-binding transcription factor 2; Processed sterol regulatory element-binding protein 2; SREBF2; BHLHD2; SREBP2; anti-SREBF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This SREBF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-427 amino acids from the Central region of human SREBF2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.49 (varies by lot)
• Sequence Positions: 399-427
• Sequence Length: 1141

• Applicable Applications for anti-SREBF2 antibody: WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(SREBF2 antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SREBF2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(SREBF2 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SREBF2 antibody detected the SREBF2 protein (arrow).)

IF (Immunofluorescence)

(Fluorescent image of U251 cell stained with SREBF2 Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SREBF2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).SREBF2 immunoreactivity is localized to Nucleus and Nucleolus significantly.)

Related Product Information for anti-SREBF2 antibody

SREBPs, including SREBP-1a, SREBP-1c, and SREBP-2, constitute a family of basic helix-loop-helix (bHLH) transcription factors that play a critical role in lipid homeostasis by regulating genes involved in cholesterol and fatty acid metabolism. Each SREBP consists of three domains, including an amino-terminal transcription factor domain of ~480 amino acids, a middle hydrophobic region of ~80 amino acids containing two hydrophobic transmembrane segments, and a carboxy-terminal regulatory domain of ~590 amino acids. SREBP-2 regulates cholesterol synthesis by activating the transcription of genes for HMG-CoA reductase and other enzymes of the cholesterol synthetic pathway. SREBP-2 is ubiquitously detected in various tissues. Under basal conditions SREBP is bound to ER membranes as a glycosylated precursor protein. Upon cholesterol depletion, the protein is cleaved to its active forms (about 50-68 kDa) and translocated into the nucleus to stimulate transcription of genes involved in the uptake and synthesis of cholesterol.

Product Categories/Family for anti-SREBF2 antibody

Cancer; Cardiovascular; Metabolism

References

Gerin, I., et al. J. Biol. Chem. 285(44):33652-33661(2010) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) Marquart, T.J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27):12228-12232(2010) Najafi-Shoushtari, S.H., et al. Science 328(5985):1566-1569(2010) Pramfalk, C., et al. J. Lipid Res. 51(6):1354-1362(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 27477113
• NCBI GeneID: 6721
• NCBI Accession #: NP_004590.2
• NCBI GenBank Nucleotide #: NM_004599.3
• UniProt Accession #: Q12772; Q05BD5; Q6GTH7; Q86V36; Q9UH04
• Molecular Weight: 123688
• NCBI Official Full Name: sterol regulatory element-binding protein 2
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 2
• NCBI Official Symbol: SREBF2
• NCBI Official Synonym Symbols: SREBP2; bHLHd2; SREBP-2
• NCBI Protein Information: sterol regulatory element-binding protein 2
• UniProt Protein Name: Sterol regulatory element-binding protein 2
• UniProt Gene Name: SREBF2
• UniProt Synonym Gene Names: BHLHD2; SREBP2; SREBP-2; bHLHd2
• UniProt Entry Name: SRBP2_HUMAN