IHC (Immunohiostchemistry)
(AAA329541 at 1/100 staining Rat testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Rabbit SREBP1 Polyclonal Antibody | anti-SREBP1 antibody
SREBP1 Antibody
Gene Names
SREBF1; SREBP1; bHLHd1; SREBP-1c
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification.
Synonyms
SREBP1, Antibody; SREBP1 Antibody; ADD 1; bHLHd1; Class D basic helix-loop-helix protein 1; D630008H06; Processed sterol regulatory element-binding protein 1; SRBP1_HUMAN; SREBF 1; SREBF1; SREBP 1; SREBP 1c; SREBP-1; SREBP1; Sterol regulatory element binding protein 1; Sterol Regulatory Element Binding Transcription Factor 1/Protein 1; Sterol regulatory element binding transcription factor 1; Sterol regulatory element-binding transcription factor 1; anti-SREBP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
SREBP1 Antibody detects endogenous levels of total SREBP1.
Tissue Specificity: Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
Tissue Specificity: Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
Purity/Purification
Peptide affinity purification.
Form/Format
Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SREBP1 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human SREBP1, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
IMPORTANT
For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA329541 at 1/100 staining Rat testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA329541 at 1/100 staining Rat testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigenretrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4°Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using SREBP-1c Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, using SREBP-1c Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SREBP1 antibody
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122 kDa
NCBI Official Full Name
sterol regulatory element-binding protein 1 isoform a
NCBI Official Synonym Full Names
sterol regulatory element binding transcription factor 1
NCBI Official Symbol
SREBF1
NCBI Official Synonym Symbols
SREBP1; bHLHd1; SREBP-1c
NCBI Protein Information
sterol regulatory element-binding protein 1; SREBP-1; class D basic helix-loop-helix protein 1
UniProt Protein Name
Sterol regulatory element-binding protein 1
UniProt Gene Name
SREBF1
UniProt Synonym Gene Names
BHLHD1; SREBP1; SREBP-1; bHLHd1
UniProt Entry Name
SRBP1_HUMAN
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Product Notes
The SREBP1 srebf1 (Catalog #AAA329541) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SREBP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SREBP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SREBP1 srebf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SREBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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