WB (Western Blot)
(Western blot analysis of SREBP2 using Jurkat whole lysates.)
Rabbit SREBP2 Polyclonal Antibody | anti-SREBP2 antibody
SREBP2 Antibody
Gene Names
SREBF2; SREBP2; bHLHd2; SREBP-2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SREBP2, Antibody; SREBP2 Antibody; AI608257; bHLHd2; Class D basic helix-loop-helix protein 2; OTTHUMP00000028740; Processed sterol regulatory element-binding protein 2; SRBP2_HUMAN; SREBF 2; Srebf2; SREBF2 protein; SREBP 2; SREBP-2; SREBP2; SREBP2gc; sterol regulatory element binding factor 2; Sterol regulatory element binding protein 2; Sterol regulatory element binding transcription factor 2; Sterol regulatory element-binding transcription factor 2; anti-SREBP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SREBP2 antibody detects endogenous levels of total SREBP2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1141
Applicable Applications for anti-SREBP2 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human SREBP2
Subcellular Location
Nucleus and Endoplasmic Reticulum Membrane. Golgi Apparatus Membrane. Cytoplasmic Vesicle > COPII-Coated Vesicle Membrane. Moves from the endoplasmic reticulum to the golgi in the absence of sterols.
Tissue Specificity
Ubiquitously expressed in adult and fetal tissues.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of SREBP2 using Jurkat whole lysates.)
WB (Western Blot)
(Western blot analysis of SREBP2 using Jurkat whole lysates.)
IHC (Immunohistochemistry)
(AAA327254 at 1/100 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA327254 at 1/100 staining human testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SREBP2 antibody
Function: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
Subunit Structure: Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139.
Post-translational Modifications: At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.
Similarity: Belongs to the SREBP family.
Subunit Structure: Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139.
Post-translational Modifications: At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.
Similarity: Belongs to the SREBP family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 124 kDa
Predicted: 124 kDa
Predicted: 124 kDa
NCBI Official Full Name
sterol regulatory element-binding protein 2
NCBI Official Synonym Full Names
sterol regulatory element binding transcription factor 2
NCBI Official Symbol
SREBF2
NCBI Official Synonym Symbols
SREBP2; bHLHd2; SREBP-2
NCBI Protein Information
sterol regulatory element-binding protein 2
UniProt Protein Name
Sterol regulatory element-binding protein 2
UniProt Gene Name
SREBF2
UniProt Synonym Gene Names
BHLHD2; SREBP2; SREBP-2; bHLHd2
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Product Notes
The SREBP2 srebf2 (Catalog #AAA327254) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SREBP2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SREBP2 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SREBP2 srebf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SREBP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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