Rabbit SREBP2 Polyclonal Antibody | anti-SREBP2 antibody

Phospho-SREBP2 (Ser455) Antibody

Cat. Num. AAA329840

• Gene Names: SREBF2; SREBP2; bHLHd2; SREBP-2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (90%)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: SREBP2, Antibody; Phospho-SREBP2 (Ser455) Antibody; AI608257; bHLHd2; Class D basic helix-loop-helix protein 2; OTTHUMP00000028740; Processed sterol regulatory element-binding protein 2; SRBP2_HUMAN; SREBF 2; Srebf2; SREBF2 protein; SREBP 2; SREBP-2; SREBP2; SREBP2gc; sterol regulatory element binding factor 2; Sterol regulatory element binding protein 2; Sterol regulatory element binding transcription factor 2; Sterol regulatory element-binding transcription factor 2; anti-SREBP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (90%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-SREBP2 (Ser455) Antibody detects endogenous levels of SREBP2 only when phosphorylated at Ser455.; Tissue Specificity: Ubiquitously expressed in adult and fetal tissues.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SREBP2 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human SREBP2 around the phosphorylation site of Ser455.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.
• Subunit Structure: Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139.
• Similarity: Belongs to the SREBP family.
• Subcellular Location: Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.; Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.Nucleus.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemisry)

(At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(Staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis SREBP-2 (Phospho-Ser455) using HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Related Product Information for anti-SREBP2 antibody

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

NCBI and Uniprot Product Information

• NCBI GI #: 451330
• NCBI GeneID: 6721
• UniProt Accession #: Q12772; Q05BD5; Q6GTH7; Q86V36; Q9UH04
• Molecular Weight: 123,688 Da
• NCBI Official Full Name: sterol regulatory element binding protein-2
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 2
• NCBI Official Symbol: SREBF2
• NCBI Official Synonym Symbols: SREBP2; bHLHd2; SREBP-2
• NCBI Protein Information: sterol regulatory element-binding protein 2; class D basic helix-loop-helix protein 2; sterol regulatory element-binding transcription factor 2
• UniProt Protein Name: Sterol regulatory element-binding protein 2
• UniProt Gene Name: SREBF2
• UniProt Synonym Gene Names: BHLHD2; SREBP2; SREBP-2; bHLHd2
• UniProt Entry Name: SRBP2_HUMAN