Rabbit SRPK1 Polyclonal Antibody | anti-SRPK1 antibody

SRPK1 Antibody

Cat. Num. AAA40846

• Gene Names: SRPK1; SFRSK1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: SRPK1, Antibody; SRPK1 Antibody; SRSF protein kinase 1, Serine/arginine-rich protein-specific kinase 1, Serine/threonine-protein kinase SRPK1, SFRSK1, SR-protein-specific kinase 1; anti-SRPK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 655

• Applicable Applications for anti-SRPK1 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: SRPK1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human SRPK1.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: Mouse Liver Tissue Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

IHC (Immunohiostchemistry)

(Immunohistochemistry of SRPK1 in human testis tissue with SRPK1 antibody at 5 μg/ml.)

WB (Western Blot)

(Western blot analysis of SRPK1 in mouse heart tissue lysate with SRPK1 antibody at 1 μg/ml.)

Related Product Information for anti-SRPK1 antibody

SRPK1 and SRPK2, two Arginine/serine-rich (SR) protein-specific kinases constitute control circuits to regulate both constitutive and alternative pre-mRNA splicing and coordinate splicing with transcription in cells (1). They are highly specific for the phosphorylation of these RS proteins, and highly expressed in testis (1,2). SRPK1 plays a role in the condensation of sperm chromatin and controls the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis (3,4).

Product Categories/Family for anti-SRPK1 antibody

Cell Cycle; Homeostasis

References

Wang HY, Arden KC, Bermingham JR Jr, et al. Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes. Genomics 1999; 57:310-5. Daub H, Blencke S, Habenberger P, et al. Identification of SRPK1 and SRPK2 as the major cellular protein kinases phosphorylating hepatitis B virus core protein. J. Virol. 2002; 76:8124-37. Zhong XY, Ding JH, Adams JA, et al. Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones. Genes Dev. 2009; 23:482-95. Mavrou A, Brakspear K, Hamdollah-Zadeh M, et al. Serine-arginine protein kinase 1 (SRPK1) inhibition as a potential novel targeted therapeutic strategy in prostate cancer. Oncogene 2014 Nov 10. doi: 10.1038/onc.2014.360.

NCBI and Uniprot Product Information

• NCBI GI #: 47419936
• NCBI GeneID: 6732
• NCBI Accession #: NP_003128
• NCBI GenBank Nucleotide #: NM_003137.4
• UniProt Accession #: Q96SB4; Q12890; Q5R364; Q5R365; Q8IY12; B4DS61
• Molecular Weight: Predicted: 54, 72, 91 kDa; Observed: 91 kDa
• NCBI Official Full Name: SRSF protein kinase 1
• NCBI Official Synonym Full Names: SRSF protein kinase 1
• NCBI Official Symbol: SRPK1
• NCBI Official Synonym Symbols: SFRSK1
• NCBI Protein Information: SRSF protein kinase 1
• UniProt Protein Name: SRSF protein kinase 1
• UniProt Gene Name: SRPK1
• UniProt Synonym Gene Names: SR-protein-specific kinase 1
• UniProt Entry Name: SRPK1_HUMAN