SRPK1 recombinant protein

Recombinant Human SRPK1 Protein (His & GST tag)

Cat. Num. AAA22091

• Gene Names: SRPK1; SFRSK1
• Purity: > 80 % as determined by SDS-PAGE
• Synonyms: SRPK1; N/A; Recombinant Human SRPK1 Protein (His & GST tag); RP3-422H11.1, SFRSK1; SRPK1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 80 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% Glycerol, 0.1mM EDTA, 0.5 mM PMSF, 1 mM TCEP, pH7.5, 5% Trehalose, 5% Mannitol, 0.01% Tween-80
• Sequence Length: 655

• Species: Human
• Sequence: Glu 2-Ser 655
• Tag: N-His-GST
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 degree C to -80 degree C.; Reconstituted protein solution can be stored at 4 degree C to 8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

SDS-PAGE

(>80% as determined by reducing SDS-PAGE)

Related Product Information for SRPK1 recombinant protein

Background: Serine / threonine-protein kinase SRPK1, also known as SFRS protein kinase 1, Serine/arginine-rich protein-specific kinase 1, SR-protein-specific kinase 1 and SRPK1, is a cytoplasm and nucleus protein which belongs to the protein kinase superfamily and CMGC Ser/Thr protein kinase family. Isoform 2 of SRPK1 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 of SRPK1 is only seen in the testis, at lower levels than isoform 2. SRPK1 hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. SRPK1 plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. SRPK1 locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. SRPK1 appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

NCBI and Uniprot Product Information

• NCBI GI #: 23468345
• NCBI GeneID: 6732
• Molecular Weight: Molecular Mass: 102 kDa; AP Molecular Mass: 120 kDa
• NCBI Official Full Name: SFRS protein kinase 1
• NCBI Official Synonym Full Names: SRSF protein kinase 1
• NCBI Official Symbol: SRPK1
• NCBI Official Synonym Symbols: SFRSK1
• NCBI Protein Information: SRSF protein kinase 1
• UniProt Protein Name: SRSF protein kinase 1
• UniProt Gene Name: SRPK1
• UniProt Synonym Gene Names: SR-protein-specific kinase 1