WB (Western Blot)
(Figure 3. Western blot analysis of STAT1 using anti- STAT1 antibody (AAA45137).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: rat thymus tissue lysates,Lane 3: rat testis tissue lysates,Lane 4: mouse testis tissue lysates,Lane 5: mouse kidney tissue lysates,Lane 6: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- STAT1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.)
Rabbit STAT1 Polyclonal Antibody | anti-STAT1 antibody
Anti-STAT1 antibody
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
STAT1, Antibody; Anti-STAT1 antibody; Signal transducer and activator of transcription 1; signal transducer and activator of transcription 1, 91kDa; Signal transducer and activator of transcription 1 91kD antibody; DKFZp686B04100 antibody; ISGF 3 antibody; ISGF-3 antibody; OTTHUMP00000163552 antibody; OTTHUMP00000165046 antibody; OTTHUMP00000165047 antibody; OTTHUMP00000205845 antibody; Signal transducer and activator of transcription 1 91kDa antibody; Signal transducer and activator of transcription 1 alpha/beta antibody; Signal transducer and activator of transcription 1 antibody; Signal transducer and activator of transcription 1, 91kD antibody; Signal transducer and activator of transcription 1-alpha/beta antibody; Signal Transductor and Activator of Transcription 1 antibody; STAT 1 antibody; STAT 91 antibody; Stat1 antibody; STAT1_HUMAN antibody; STAT91 antibody; Transcription factor ISGF 3 components p91 p84 antibody; Transcription factor ISGF-3 components p91/p84 antibody; anti-STAT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
750
Applicable Applications for anti-STAT1 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human STAT1 (364-378aa FDKDVNERNTVKGFR), different from the related mouse sequence by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 3. Western blot analysis of STAT1 using anti- STAT1 antibody (AAA45137).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: rat thymus tissue lysates,Lane 3: rat testis tissue lysates,Lane 4: mouse testis tissue lysates,Lane 5: mouse kidney tissue lysates,Lane 6: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- STAT1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.)
WB (Western Blot)
(Figure 3. Western blot analysis of STAT1 using anti- STAT1 antibody (AAA45137).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: rat thymus tissue lysates,Lane 3: rat testis tissue lysates,Lane 4: mouse testis tissue lysates,Lane 5: mouse kidney tissue lysates,Lane 6: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- STAT1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT1 at approximately 91KD. The expected band size for STAT1 is at 87KD.)
WB (Western Blot)
(Anti-STAT1 antibody, AAA45137, Western blottingLane 1: Mouse Heart Tissue LysateLane 2: Mouse Liver Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Kidney Tissue LysateLane 5: Mouse Spleen Tissue LysateLane 6: Mouse Thymus Tissue LysateLane 7: Mouse Lung Tissue LysateLane 8: Mouse Intestine Tissue LysateLane 9: Mouse Ovary Tissue Lysate)
WB (Western Blot)
(Anti-STAT1 antibody, AAA45137, Western blottingLane 1: Mouse Heart Tissue LysateLane 2: Mouse Liver Tissue LysateLane 3: Mouse Brain Tissue LysateLane 4: Mouse Kidney Tissue LysateLane 5: Mouse Spleen Tissue LysateLane 6: Mouse Thymus Tissue LysateLane 7: Mouse Lung Tissue LysateLane 8: Mouse Intestine Tissue LysateLane 9: Mouse Ovary Tissue Lysate)
WB (Western Blot)
(Anti-STAT1 antibody, AAA45137, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell Lysate)
WB (Western Blot)
(Anti-STAT1 antibody, AAA45137, Western blottingLane 1: MCF-7 Cell LysateLane 2: HELA Cell Lysate)
Related Product Information for anti-STAT1 antibody
Description: Rabbit IgG polyclonal antibody for Signal transducer and activator of transcription 1-alpha/beta(STAT1) detection. Tested with WB in Human, Mouse, Rat.
Background: Chen et al.(1998) determined the crystal structure of the DNA complex of a 67-kD core fragment of the STAT1 homodimer, lacking only the N-domain and the C-terminal transcriptional activation domain, at 2.9-angstrom resolution. Phosphorylation of Signal Transducer and Activator of transcription 1(STAT 1) was also decreased in rheumatoid arthritis lymphocytes. The transcription factor signal transducer and activator of transcription-1(STAT1) plays a key role in immunity against mycobacterial and viral infections. Activation of the signal transducers and activators of transcription(STAT) pathway is important in fibroblast growth factor(FGF) modulation of chondrocyte proliferation and endochondral bone formation during embryogenesis.
Background: Chen et al.(1998) determined the crystal structure of the DNA complex of a 67-kD core fragment of the STAT1 homodimer, lacking only the N-domain and the C-terminal transcriptional activation domain, at 2.9-angstrom resolution. Phosphorylation of Signal Transducer and Activator of transcription 1(STAT 1) was also decreased in rheumatoid arthritis lymphocytes. The transcription factor signal transducer and activator of transcription-1(STAT1) plays a key role in immunity against mycobacterial and viral infections. Activation of the signal transducers and activators of transcription(STAT) pathway is important in fibroblast growth factor(FGF) modulation of chondrocyte proliferation and endochondral bone formation during embryogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
83,043 Da
NCBI Official Full Name
Signal transducer and activator of transcription 1-alpha/beta
NCBI Official Synonym Full Names
signal transducer and activator of transcription 1, 91kDa
NCBI Official Symbol
STAT1
NCBI Official Synonym Symbols
CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
NCBI Protein Information
signal transducer and activator of transcription 1-alpha/beta; transcription factor ISGF-3 components p91/p84; signal transducer and activator of transcription-1; signal transducer and activator of transcription 1, 91kD
UniProt Protein Name
Signal transducer and activator of transcription 1-alpha/beta
UniProt Gene Name
STAT1
UniProt Entry Name
STAT1_HUMAN
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Product Notes
The STAT1 stat1 (Catalog #AAA45137) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-STAT1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's STAT1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the STAT1 stat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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