DB (Dot Blot)
(STAT2 phospho Tyr689 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of STAT2 phospho Tyr689 pAb for STAT2 phospho Tyr689. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.)
Rabbit anti-Human STAT2 phospho Tyr689 Polyclonal Antibody | anti-STAT2 antibody
STAT2 phospho Tyr689 antibody (pAb)
Gene Names
STAT2; P113; ISGF-3; STAT113
Reactivity
Human
Applications
Western Blot, Dot Blot
Purity
Affinity Purified
Synonyms
STAT2 phospho Tyr689, Antibody; STAT2 phospho Tyr689 antibody (pAb); P113; ISGF-3; STAT113; anti-STAT2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-STAT2 antibody
WB (Western Blot), DB (Dot Blot)
Conjugation
Phosphorylated
Immunogen
This STAT2 phospho Tyr689 antibody was raised against a peptide containing phospho Tyr689 of human STAT2.
Abs Residue
Tyr
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(STAT2 phospho Tyr689 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of STAT2 phospho Tyr689 pAb for STAT2 phospho Tyr689. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.)
DB (Dot Blot)
(STAT2 phospho Tyr689 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of STAT2 phospho Tyr689 pAb for STAT2 phospho Tyr689. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.)
WB (Western Blot)
(STAT2 phospho Tyr689 pAb tested by Western blot. HeLa whole-cell extract (20 ug per lane) was probed with STAT2 phospho Tyr689 pAb (1:500). Lane 1: No treatment. Lane 2: Cells treated with IFNa (2500 units/ml for 30 minutes).)
WB (Western Blot)
(STAT2 phospho Tyr689 pAb tested by Western blot. HeLa whole-cell extract (20 ug per lane) was probed with STAT2 phospho Tyr689 pAb (1:500). Lane 1: No treatment. Lane 2: Cells treated with IFNa (2500 units/ml for 30 minutes).)
Related Product Information for anti-STAT2 antibody
Short Description: Our STAT2 phospho Tyr689 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Dot blot and Western blot; it has been shown to react with Human samples.
Background: STAT2 (signal transducer and activator of transcription 2) is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT2 has been shown to be activated by IFNa and IFNb, and to associate with the transcription factors p48 and p300.
Background: STAT2 (signal transducer and activator of transcription 2) is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT2 has been shown to be activated by IFNa and IFNb, and to associate with the transcription factors p48 and p300.
Product Categories/Family for anti-STAT2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
97,457 Da
NCBI Official Full Name
signal transducer and activator of transcription 2 isoform 1
NCBI Official Synonym Full Names
signal transducer and activator of transcription 2, 113kDa
NCBI Official Symbol
STAT2
NCBI Official Synonym Symbols
P113; ISGF-3; STAT113
NCBI Protein Information
signal transducer and activator of transcription 2; interferon alpha induced transcriptional activator; signal transducer and activator of transcription 2, 113kD
UniProt Protein Name
Signal transducer and activator of transcription 2
UniProt Gene Name
STAT2
UniProt Entry Name
STAT2_HUMAN
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Product Notes
The STAT2 stat2 (Catalog #AAA59893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STAT2 phospho Tyr689 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STAT2 phospho Tyr689 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), DB (Dot Blot). Researchers should empirically determine the suitability of the STAT2 stat2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT2 phospho Tyr689, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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