WB (Western Blot)
(Dilution: WB - 1:2000Anti-STAT3(S727) Antibody at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse STAT3 Polyclonal Antibody | anti-STAT3 antibody
STAT3 (S727) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
STAT3, Antibody; STAT3 (S727) Antibody; Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF; anti-STAT3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This STAT3(S727) antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 710-740 amino acids from the human region of human STAT3(S727).
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
769
Applicable Applications for anti-STAT3 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthesized Peptide
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Dilution: WB - 1:2000Anti-STAT3(S727) Antibody at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB - 1:2000Anti-STAT3(S727) Antibody at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB - 1:500Anti-STAT3(S727) Antibody at 1:500 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Dilution: WB - 1:500Anti-STAT3(S727) Antibody at 1:500 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 88 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-STAT3 antibody
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)- responsive elements identified in the promoters of various acute- phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity).
Product Categories/Family for anti-STAT3 antibody
References
Akira S.,et al.Cell 77:63-71(1994). Della Pietra L.,et al.Gene 213:119-124(1998). Feinstein E.,et al.Patent number EP2440214, 18-APR-2012. Ota T.,et al.Nat. Genet. 36:40-45(2004). Zody M.C.,et al.Nature 440:1045-1049(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88068 Da
NCBI Official Full Name
signal transducer and activator of transcription 3 isoform 2
NCBI Official Synonym Full Names
signal transducer and activator of transcription 3
NCBI Official Symbol
STAT3
NCBI Official Synonym Symbols
APRF; HIES; ADMIO; ADMIO1
NCBI Protein Information
signal transducer and activator of transcription 3
UniProt Protein Name
Signal transducer and activator of transcription 3
UniProt Gene Name
STAT3
UniProt Synonym Gene Names
APRF
UniProt Entry Name
STAT3_HUMAN
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Product Notes
The STAT3 stat3 (Catalog #AAA290931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The STAT3 (S727) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's STAT3 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the STAT3 stat3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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