IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA286825 STAT3 (C-term S727) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the STAT3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).)
Rabbit anti-Human STAT3 Polyclonal Antibody | anti-STAT3 antibody
STAT3 Antibody (C-term S727)
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
STAT3, Antibody; STAT3 Antibody (C-term S727); Signal transducer and activator of transcription 3; Acute-phase response factor; STAT3; APRF; anti-STAT3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This STAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide from the C-terminal region of human STAT3, aa 711-743.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
711-743
Sequence Length
722
Applicable Applications for anti-STAT3 antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA286825 STAT3 (C-term S727) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the STAT3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).)
IF (Immunofluorescence)
(Fluorescent confocal image of SY5Y cells stained with STAT3 (C-term S727) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA286825 STAT3 (C-term S727) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the STAT3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000168610).)
WB (Western Blot)
(STAT3 Antibody (pS727) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the STAT3 antibody detected the STAT3 protein (arrow).)
WB (Western Blot)
(STAT3 Antibody (pS727) western blot analysis in Daudi cell line lysates (35ug/lane).This demonstrates the STAT3 antibody detected the STAT3 protein (arrow).)
Related Product Information for anti-STAT3 antibody
The protein encoded by this gene is a member of the STAT
protein family. In response to cytokines and growth factors, STAT
family members are phosphorylated by the receptor associated
kinases, and then form homo- or heterodimers that translocate to
the cell nucleus where they act as transcription activators. This
protein is activated through phosphorylation in response to various
cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF,
LIF and BMP2. This protein mediates the expression of a variety of
genes in response to cell stimuli, and thus plays a key role in
many cellular processes such as cell growth and apoptosis. The
small GTPase Rac1 has been shown to bind and regulate the activity
of this protein. PIAS3 protein is a specific inhibitor of this
protein. Three alternatively spliced transcript variants encoding
distinct isoforms have been described.
protein family. In response to cytokines and growth factors, STAT
family members are phosphorylated by the receptor associated
kinases, and then form homo- or heterodimers that translocate to
the cell nucleus where they act as transcription activators. This
protein is activated through phosphorylation in response to various
cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF,
LIF and BMP2. This protein mediates the expression of a variety of
genes in response to cell stimuli, and thus plays a key role in
many cellular processes such as cell growth and apoptosis. The
small GTPase Rac1 has been shown to bind and regulate the activity
of this protein. PIAS3 protein is a specific inhibitor of this
protein. Three alternatively spliced transcript variants encoding
distinct isoforms have been described.
Product Categories/Family for anti-STAT3 antibody
References
References for protein:
1.Li, L., et al. Cancer Res. 70(20):8222-8232(2010)
2Takaishi, K., et al. Cancer Sci. 101(10):2128-2136(2010)
3.Li, F., et al. Surgeon 8(5):262-266(2010)
4.Schimke, L.F., et al. J. Allergy Clin. Immunol. 126(3):611-617(2010)
5.Iliopoulos, D., et al. Mol. Cell 39(4):493-506(2010)
References for SY5Y (SH-SY5Y; #CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
1.Li, L., et al. Cancer Res. 70(20):8222-8232(2010)
2Takaishi, K., et al. Cancer Sci. 101(10):2128-2136(2010)
3.Li, F., et al. Surgeon 8(5):262-266(2010)
4.Schimke, L.F., et al. J. Allergy Clin. Immunol. 126(3):611-617(2010)
5.Iliopoulos, D., et al. Mol. Cell 39(4):493-506(2010)
References for SY5Y (SH-SY5Y; #CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88068
NCBI Official Full Name
signal transducer and activator of transcription 3 isoform 2
NCBI Official Synonym Full Names
signal transducer and activator of transcription 3 (acute-phase response factor)
NCBI Official Symbol
STAT3
NCBI Official Synonym Symbols
APRF; HIES; ADMIO
NCBI Protein Information
signal transducer and activator of transcription 3
UniProt Protein Name
Signal transducer and activator of transcription 3
UniProt Gene Name
STAT3
UniProt Synonym Gene Names
APRF
UniProt Entry Name
STAT3_HUMAN
Similar Products
Product Notes
The STAT3 stat3 (Catalog #AAA286825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 711-743. The STAT3 Antibody (C-term S727) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STAT3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the STAT3 stat3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.