Rabbit Surfactant protein D Polyclonal Antibody | anti-SFTPD antibody

Surfactant protein D Antibody

Cat. Num. AAA329122

• Gene Names: SFTPD; SP-D; PSP-D; SFTP4; COLEC7
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Surfactant protein D, Antibody; Surfactant protein D Antibody; COLEC 7; COLEC7; Collectin-7; Collectin7; Lung surfactant protein D; PSP D; PSP-D; PSP-D Surfactant protein D; PSPD; Pulmonary surfactant apoprotein; Pulmonary surfactant associated protein D; Pulmonary surfactant associated protein PSP-D; Pulmonary surfactant-associated protein D; SFTP 4; SFTP4; SFTPD; SFTPD_HUMAN; SP D; SP-D; Surfactant associated protein pulmonary 4; Surfactant protein D; Surfactant pulmonary associated protein D; anti-SFTPD antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Surfactant protein D Antibody detects endogenous levels of total Surfactant protein D.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 375

• Applicable Applications for anti-SFTPD antibody: ELISA
• Immunogen: A synthesized peptide derived from human Surfactant protein D, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IF (Immunofluorescence)

(AAA329122 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329122 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

WB (Western Blot)

(Western blot analysis of extracts from 3T3, using Surfactant protein D Antibody. The lane on the left was treated with blocking peptide.)

WB (Western Blot)

(Western blot analysis of extracts from Rat lung, using Surfactant protein D Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-SFTPD antibody

Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Post Translational Modifications: The N-terminus is blocked.Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.S-nitrosylation at Cys-35 and Cys-40 alters the quaternary structure which results in a pro-inflammatory chemoattractive signaling activity with macrophages.

Subcellular Location: Secreted>Extracellular space>Extracellular matrix. Secreted>Extracellular space>Surface film.

Tissue Specificity: Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes).

Subunit Structure: Oligomeric complex of 4 set of homotrimers.

Similarity: Belongs to the SFTPD family.

NCBI and Uniprot Product Information

• NCBI GI #: 61699226
• NCBI GeneID: 6441
• NCBI Accession #: NP_003010.4
• NCBI GenBank Nucleotide #: NP_003010.4
• UniProt Accession #: P35247
• Molecular Weight: Observed Molecular Weight: (Observed)38kD.; Predicted Molecular Weight: (Calculated)38kDa.
• NCBI Official Full Name: pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: SFTPD
• NCBI Official Synonym Symbols: SP-D; PSP-D; SFTP4; COLEC7
• NCBI Protein Information: pulmonary surfactant-associated protein D
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• UniProt Gene Name: SFTPD
• UniProt Synonym Gene Names: COLEC7; PSPD; SFTP4; PSP-D; SP-D
• UniProt Entry Name: SFTPD_HUMAN