Rabbit SYVN1 / HRD1 Polyclonal Antibody | anti-SYVN1 antibody

Rabbit Polyclonal to Human SYVN1 / HRD1

Cat. Num. AAA52323

• Gene Names: SYVN1; DER3; HRD1
• Reactivity: Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat, Zebrafish
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: SYVN1 / HRD1, Antibody; Rabbit Polyclonal to Human SYVN1 / HRD1; Anti-SYVN1 / HRD1 Antibody (Internal) IHC-plus; SYVN1; KIAA1810; Synoviolin; Synovial apoptosis inhibitor 1; DER3; HRD1; Human SYVN1; anti-SYVN1 antibody

• Host: Rabbit
• Reactivity: Bat, Gibbon, Bovine, Dog, Xenopus, Gorilla, Hamster, Horse, Human, Monkey, Mouse, Rabbit, Rat, Zebrafish
• Clonality: Polyclonal
• Specificity: Human SYVN1 / HRD1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 616

• Applicable Applications for anti-SYVN1 antibody: IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: Synthetic 18 amino acid peptide from internal region of human SYVN1 / HRD1.
• Antigen Type: Synthetic peptide
• Immunogen: SYVN1 / HRD1 antibody was raised against synthetic 18 amino acid peptide from internal region of human SYVN1 / HRD1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Opossum, Xenopus, Stickleback, Pufferfish, Zebrafish (100%).
• Antigen Modification: Internal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to one month. Avoid freeze-thaw cycles.

IHC (Immunohistochemistry)

(Anti-SYVN1 / HRD1 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

NCBI and Uniprot Product Information

• NCBI GI #: 27436925
• NCBI GeneID: 84447
• NCBI Accession #: NP_115807.1
• NCBI GenBank Nucleotide #: NM_032431.2
• UniProt Accession #: Q86TM6; Q8N3K3; Q8N6E8; Q96JL5; Q96PK3
• NCBI Official Full Name: E3 ubiquitin-protein ligase synoviolin isoform a
• NCBI Official Synonym Full Names: synovial apoptosis inhibitor 1, synoviolin
• NCBI Official Symbol: SYVN1
• NCBI Official Synonym Symbols: DER3; HRD1
• NCBI Protein Information: E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog
• UniProt Protein Name: E3 ubiquitin-protein ligase synoviolin
• UniProt Gene Name: SYVN1
• UniProt Synonym Gene Names: HRD1; KIAA1810
• UniProt Entry Name: SYVN1_HUMAN