FCM/FACS (Flow Cytometry)
(AAA61704 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Goat SYVN1 Polyclonal Antibody | anti-SYVN1 antibody
Goat anti-SYVN1 Antibody
Gene Names
SYVN1; DER3; HRD1
Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
SYVN1, Antibody; Goat anti-SYVN1 Antibody; SYVN1; synovial apoptosis inhibitor 1, synoviolin; HRD1; KIAA1810; MGC40372; synoviolin 1; HRD1 antibody; KIAA1810 antibody; MGC40372 antibody; synovial apoptosis inhibitor 1; synoviolin antibody; synoviolin 1 antibody; SYVN1 antibody; anti-SYVN1 antibody
Host
Goat
Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence Length
616
Applicable Applications for anti-SYVN1 antibody
WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-HERQHLEARLQS, from the internal region of the protein sequence according to NP_115807.1; NP_757385.1.
Note
This antibody is expected to recognize isoforms a and b (NP_115807.1; NP_757385.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
FCM/FACS (Flow Cytometry)
(AAA61704 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(AAA61704 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(AAA61704 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(AAA61704 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
WB (Western Blot)
((2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
((2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
E3 ubiquitin-protein ligase synoviolin isoform a
NCBI Official Synonym Full Names
synoviolin 1
NCBI Official Symbol
SYVN1
NCBI Official Synonym Symbols
DER3; HRD1
NCBI Protein Information
E3 ubiquitin-protein ligase synoviolin
UniProt Protein Name
E3 ubiquitin-protein ligase synoviolin
UniProt Gene Name
SYVN1
UniProt Synonym Gene Names
HRD1; KIAA1810
UniProt Entry Name
SYVN1_HUMAN
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Product Notes
The SYVN1 syvn1 (Catalog #AAA61704) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SYVN1 Antibody reacts with Tested: Human, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's SYVN1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the SYVN1 syvn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYVN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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