IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with TADA3L Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TADA3L primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.)
Rabbit TADA3L Polyclonal Antibody | anti-TADA3 antibody
TADA3L Antibody (C-term)
Gene Names
TADA3; ADA3; NGG1; hADA3; STAF54; TADA3L
Reactivity
Human (Predicted Reactivity: Xenopus, Bovine, Zebrafish, Mouse, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TADA3L, Antibody; TADA3L Antibody (C-term); Transcriptional adapter 3; ADA3 homolog; hADA3; STAF54; Transcriptional adapter 3-like; ADA3-like protein; TADA3; ADA3; TADA3L; anti-TADA3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Xenopus, Bovine, Zebrafish, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TADA3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 386-414 amino acids from the C-terminal region of human TADA3L.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
386-414
Sequence Length
432
Applicable Applications for anti-TADA3 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with TADA3L Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TADA3L primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of U251 cell stained with TADA3L Antibody (C-term). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TADA3L primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TADA3L immunoreactivity is localized to Nucleus significantly.)
WB (Western Blot)
(TADA3L Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TADA3L antibody detected the TADA3L protein (arrow).)
WB (Western Blot)
(TADA3L Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TADA3L antibody detected the TADA3L protein (arrow).)
Related Product Information for anti-TADA3 antibody
Many DNA-binding transcriptional activator proteins
enhance the initiation rate of RNA polymerase II-mediated gene
transcription by interacting functionally with the general
transcription machinery bound at the basal promoter. Adaptor
proteins are usually required for this activation, possibly to
acetylate and destabilize nucleosomes, thereby relieving chromatin
constraints at the promoter. The protein encoded by this gene is a
transcriptional activator adaptor and has been found to be part of
the PCAF histone acetylase complex. In addition, it associates with
the tumor suppressor protein p53 and is required for full activity
of p53 and p53-mediated apoptosis. At least four alternatively
spliced variants have been found for this gene, but the full-length
nature of some variants has not been determined. [provided by
RefSeq].
enhance the initiation rate of RNA polymerase II-mediated gene
transcription by interacting functionally with the general
transcription machinery bound at the basal promoter. Adaptor
proteins are usually required for this activation, possibly to
acetylate and destabilize nucleosomes, thereby relieving chromatin
constraints at the promoter. The protein encoded by this gene is a
transcriptional activator adaptor and has been found to be part of
the PCAF histone acetylase complex. In addition, it associates with
the tumor suppressor protein p53 and is required for full activity
of p53 and p53-mediated apoptosis. At least four alternatively
spliced variants have been found for this gene, but the full-length
nature of some variants has not been determined. [provided by
RefSeq].
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Li, C.W., et al. Nucleic Acids Res. 38(16):5291-5303(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Hu, Y., et al. Cancer Invest. 27(3):298-306(2009)
Wang, Y.L., et al. J. Biol. Chem. 283(49):33808-33815(2008)
Li, C.W., et al. Nucleic Acids Res. 38(16):5291-5303(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Hu, Y., et al. Cancer Invest. 27(3):298-306(2009)
Wang, Y.L., et al. J. Biol. Chem. 283(49):33808-33815(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48902
NCBI Official Full Name
transcriptional adapter 3 isoform a
NCBI Official Synonym Full Names
transcriptional adaptor 3
NCBI Official Symbol
TADA3
NCBI Official Synonym Symbols
ADA3; NGG1; hADA3; STAF54; TADA3L
NCBI Protein Information
transcriptional adapter 3
UniProt Protein Name
Transcriptional adapter 3
UniProt Gene Name
TADA3
UniProt Synonym Gene Names
ADA3; TADA3L; hADA3; ADA3-like protein
UniProt Entry Name
TADA3_HUMAN
Similar Products
Product Notes
The TADA3 tada3 (Catalog #AAA286878) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 386-414. The TADA3L Antibody (C-term) reacts with Human (Predicted Reactivity: Xenopus, Bovine, Zebrafish, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's TADA3L can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TADA3 tada3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TADA3L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.