WB (Western Blot)
(Detection of human and mouse TAF1 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, HeLa, HEK293T, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAF1 antibody AAA212196 (lot AAA212196-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human, Mouse TAF1 Polyclonal Antibody | anti-TAF1 antibody
Rabbit anti-TAF1 Antibody, Affinity Purified
Gene Names
TAF1; OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TAF1, Antibody; Rabbit anti-TAF1 Antibody, Affinity Purified; Transcription initiation factor TFIID 250 kDa subunit; TAF(II)250; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa; TAF2A; TAFII250; TAFII-250; OF; transcription factor TFIID p250 polypeptide; N-TAF1; transcription initiation factor TFIID subunit 1; XDP; TBP-associated factor 250 kDa; cell cycle gene 1 protein; BA2R; P250; CCGS; cell cycle, G1 phase defect; complementation of cell cycle block, G1-to-S; DYT3; DYT3/TAF1; KAT4; MRXS33; NSCL2; CCG1; Transcription initiation factor TFIID subunit 1; TATA-box binding protein associated factor 1; anti-TAF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TAF1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Golden hamster
Immunogen
Between 1800 and 1850
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse TAF1 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, HeLa, HEK293T, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAF1 antibody AAA212196 (lot AAA212196-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human and mouse TAF1 by western blot. Samples: Whole cell lysate (15 ug) from Jurkat, HeLa, HEK293T, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAF1 antibody AAA212196 (lot AAA212196-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human TAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAF1 antibody AAA212196 (lot AAA212196-2) used for IP at 6 ug per reaction. TAF1 was also immunoprecipitated by a previous lot of this antibody (lot AAA212196-1) and rabbit anti-TAF1 antibody For blotting immunoprecipitated TAF1, AAA212196 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human TAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TAF1 antibody AAA212196 (lot AAA212196-2) used for IP at 6 ug per reaction. TAF1 was also immunoprecipitated by a previous lot of this antibody (lot AAA212196-1) and rabbit anti-TAF1 antibody For blotting immunoprecipitated TAF1, AAA212196 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-TAF1 antibody
Transcription initiation factor TFIID subunit 1 (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. It also phosphorylates GTF2A1 and GTF2F1 on Ser residues. TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P21675].
Product Categories/Family for anti-TAF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~130 kDa
NCBI Official Full Name
Homo sapiens TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa (TAF1), transcript variant 2, mRNA
NCBI Official Synonym Full Names
TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
NCBI Official Symbol
TAF1
NCBI Official Synonym Symbols
OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1
NCBI Protein Information
transcription initiation factor TFIID subunit 1; TAFII-250; TAF(II)250; cell cycle gene 1 protein; cell cycle, G1 phase defect; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; complementation of cell cycle block, G1-to-S; trans
UniProt Protein Name
Transcription initiation factor TFIID subunit 1
UniProt Gene Name
TAF1
UniProt Synonym Gene Names
BA2R; CCG1; CCGS; TAF2A; p250; TAF(II)250; TAFII-250; TAFII250
UniProt Entry Name
TAF1_HUMAN
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Product Notes
The TAF1 taf1 (Catalog #AAA212196) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TAF1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TAF1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TAF1 taf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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