Application Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
TAF1 recombinant protein
Recombinant TAF1 (1522-1656) protein
Gene Names
TAF1; OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1
Synonyms
TAF1; N/A; Recombinant TAF1 (1522-1656) protein; TATA-Box Binding Protein Associated Factor 1; Cell Cycle Gene 1 Protein; TAF2A; TAF1 recombinant protein
Host
E Coli
Form/Format
Recombinant TAF1 (1522-1656) protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 10% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant TAF1 (1522-1656) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
Application Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
Application Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
Application Data
(Recombinant TAF1 (1522-1656) protein activity assay. 3 uM histone peptide H3K18ac peptide was incubated with Recombinant TAF1 (1522-1656) protein in a reaction system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour at room temperature. DYKDDDDK antibody and SA-XL665 (each at 1:100 in reaction buffer) was added for 30 min. All operations were performed at RT. HTRF assay was used for detection of reaction products.)
SDS-PAGE
(Recombinant TAF1 (1522-1656) protein gel. TAF1 (1522-1656) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.3 kDa Purity: ? 90%)
SDS-PAGE
(Recombinant TAF1 (1522-1656) protein gel. TAF1 (1522-1656) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 19.3 kDa Purity: ? 90%)
Related Product Information for TAF1 recombinant protein
Short Description: The peptide corresponding to amino acids 1522-1656 that contains the bromodomain sequences of TAF1 (accession number NP_004597.2) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 21.75 kDa. It shows binding specificity for acetylated H3K14. TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 also contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1 has been shown to phosphorylate threonine 55 of p53 which leads to MDM2-mediated degradation of p53. TAF1 also phosphorylates GTF2A1 and GTF2F1 on serine residues. In addition, TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle. Recombinant TAF1 (1522-1656) can be used in binding assays and inhibitor screening.
Background: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 also contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1 has been shown to phosphorylate threonine 55 of p53 which leads to MDM2-mediated degradation of p53. TAF1 also phosphorylates GTF2A1 and GTF2F1 on serine residues. In addition, TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle.
Background: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 also contains bromodomains for interaction with other proteins. The bromodomain functions as a 'reader' of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1 has been shown to phosphorylate threonine 55 of p53 which leads to MDM2-mediated degradation of p53. TAF1 also phosphorylates GTF2A1 and GTF2F1 on serine residues. In addition, TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle.
Product Categories/Family for TAF1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
212,677 Da
NCBI Official Full Name
transcription initiation factor TFIID subunit 1 isoform 1
NCBI Official Synonym Full Names
TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
NCBI Official Symbol
TAF1
NCBI Official Synonym Symbols
OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1
NCBI Protein Information
transcription initiation factor TFIID subunit 1; TAFII-250; TAF(II)250; cell cycle gene 1 protein; cell cycle, G1 phase defect; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; complementation of cell cycle block, G1-to-S; trans
UniProt Protein Name
Transcription initiation factor TFIID subunit 1
UniProt Gene Name
TAF1
UniProt Synonym Gene Names
BA2R; CCG1; CCGS; TAF2A; p250; TAF(II)250; TAFII-250; TAFII250
UniProt Entry Name
TAF1_HUMAN
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Product Notes
The TAF1 taf1 (Catalog #AAA59684) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TAF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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