FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of K562 cells using anti-TAPA1 antibody (AAA124636).Overlay histogram showing K562 cells stained with AAA124636 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (AAA124636,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit TAPA1 Polyclonal Antibody | anti-TAPA1 antibody
Anti-TAPA1 Picoband antibody
No cross reactivity with other proteins.
No cross reactivity with other proteins.
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of K562 cells using anti-TAPA1 antibody (AAA124636).Overlay histogram showing K562 cells stained with AAA124636 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (AAA124636,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 4. Flow Cytometry analysis of K562 cells using anti-TAPA1 antibody (AAA124636).Overlay histogram showing K562 cells stained with AAA124636 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (AAA124636,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Jurkat cells using anti-TAPA1 antibody (AAA124636).Overlay histogram showing Jurkat cells stained with AAA124636 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (AAA124636,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 3. Flow Cytometry analysis of Jurkat cells using anti-TAPA1 antibody (AAA124636).Overlay histogram showing Jurkat cells stained with AAA124636 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (AAA124636,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PBMC cells using anti-TAPA1 antibody (AAA124636).Overlay histogram showing PBMC cells stained with AAA124636 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (AAA124636,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of PBMC cells using anti-TAPA1 antibody (AAA124636).Overlay histogram showing PBMC cells stained with AAA124636 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TAPA1 Antibody (AAA124636,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of TAPA1 using anti-TAPA1 antibody (AAA124636).TAPA1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAPA1 Antibody (AAA124636) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 1. IHC analysis of TAPA1 using anti-TAPA1 antibody (AAA124636).TAPA1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TAPA1 Antibody (AAA124636) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Protein Function: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.
NCBI and Uniprot Product Information
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Product Notes
The TAPA1 cd81 (Catalog #AAA124636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TAPA1 Picoband antibody reacts with Human, Mouse, Rat No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's TAPA1 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the TAPA1 cd81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TAPA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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