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Knockout Validation
(Knockout Validation: Lane 1: Wild-type U87MG cell lysate; Lane 2: TAPA1 knockout U87MG cell lysate; Predicted MW: 26kDa Observed MW: 29kDa Primary Ab: 3ug/ml Rabbit Anti-Human TAPA1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit anti-Human Target Of The Antiproliferative Antibody 1 Polyclonal Antibody | anti-TAPA1 antibody
Target Of The Antiproliferative Antibody 1 (TAPA1) Polyclonal Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Synonyms
Target Of The Antiproliferative Antibody 1, Antibody; Target Of The Antiproliferative Antibody 1 (TAPA1) Polyclonal Antibody; CD81; S5.7; TAPA1; TSPAN28; Tetraspanin 28; Target Of Antiproliferative Antibody 1; 26 kDa cell surface protein TAPA-1; anti-TAPA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TAPA1. It has been selected for its ability to recognize TAPA1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
0.77mg/ml (varies by lot)
Sequence Length
22
Applicable Applications for anti-TAPA1 antibody
ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human
Organism Species
Human
Fragment
TAPA1 (Phe113~Lys201)
Quality Control
Content: The quality control contains recombinant TAPA1 (Phe113~Lys201) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Knockout Validation
(Knockout Validation: Lane 1: Wild-type U87MG cell lysate; Lane 2: TAPA1 knockout U87MG cell lysate; Predicted MW: 26kDa Observed MW: 29kDa Primary Ab: 3ug/ml Rabbit Anti-Human TAPA1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Knockout Validation
(Knockout Validation: Lane 1: Wild-type U87MG cell lysate; Lane 2: TAPA1 knockout U87MG cell lysate; Predicted MW: 26kDa Observed MW: 29kDa Primary Ab: 3ug/ml Rabbit Anti-Human TAPA1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistochemisry)
(DAB staining on IHC-P. Samples: Human Tissue))
IHC (Immunohistochemisry)
(DAB staining on IHC-P. Samples: Human Tissue))
WB (Western Blot)
(Western Blot: Sample: Recombinant TAPA1, Human.)
WB (Western Blot)
(Western Blot: Sample: Recombinant TAPA1, Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Lymphocytes Cells; Lane2: Human U-87MG Cells; Lane3: Mouse RAW 264.7 Cells.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human Lymphocytes Cells; Lane2: Human U-87MG Cells; Lane3: Mouse RAW 264.7 Cells.)
Product Categories/Family for anti-TAPA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,809 Da
NCBI Official Full Name
CD81 antigen isoform 2
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
CD81
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
NCBI Protein Information
CD81 antigen
UniProt Protein Name
CD81 antigen
UniProt Gene Name
CD81
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28
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Product Notes
The TAPA1 cd81 (Catalog #AAA144891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Target Of The Antiproliferative Antibody 1 (TAPA1) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Target Of The Antiproliferative Antibody 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TAPA1 cd81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Target Of The Antiproliferative Antibody 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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