Rabbit tbx21 Polyclonal Antibody | anti-tbx21 antibody

tbx21 Antibody

Cat. Num. AAA327315

• Gene Names: TBX21; TBET; T-PET; T-bet; TBLYM
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: tbx21, Antibody; tbx21 Antibody; T bet; T box 21; T box expressed in T cells; T box protein 21; T box transcription factor TBX21; T cell specific T box transcription factor; T cell specific T box transcription factor T bet; T PET; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; TBET; TBLYM; TBX 21; Tbx21; TBX21_HUMAN; TPET; Transcription factor TBLYM; anti-tbx21 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: tbx21 antibody detects endogenous levels of total tbx21
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 535

• Applicable Applications for anti-tbx21 antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human tbx21
• Subcellular Location: Nucleus.
• Tissue Specificity: T-cell specific.
• Predicted Cross Reactivity: Pig, Bovine, Sheep, Rabbit, Dog
• Similarity: Pig (100%), Bovine (91%), Sheep (100%), Rabbit (100%), Dog (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemisry)

(AAA327315 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence)

(AAA327315 staining NK92 cells by ICC/IF.)

WB (Western Blot)

(Western blot analysis of tbx21 using HuvEc whole lysates.)

Related Product Information for anti-tbx21 antibody

Function: Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931). Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation (By similarity). Can activate Th1 genes also via recruitment of Mediator complex and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1) in the form of the super elongation complex (SEC) to super-enhancers and associated genes in activated Th1 cells (PubMed:27292648). Inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. Inhibits the Th2 cell lineage commitment by suppressing the production of Th2 cytokines, such as IL-4, IL-5, and IL-13, via repression of transcriptional regulators GATA3 and NFATC2. Protects Th1 cells from amplifying aberrant type-I IFN response in an IFN-gamma abundant microenvironment by acting as a repressor of type-I IFN transcription factors and type-I IFN-stimulated genes. Acts as a regulator of antiviral B-cell responses; controls chronic viral infection by promoting the antiviral antibody IgG2a isotype switching and via regulation of a broad antiviral gene expression program (By similarity).
Subunit Structure: Interacts with RUNX1, RUNX3, ITK, ABL1, RELA, CDK9 and KDM6B. The phosphorylated form (at Thr-303) interacts with NFATC2. Interacts with SMARCA4 in a KDM6B-dependent manner (By similarity). Interacts with CCTN1 (PubMed:27292648). Interacts with USP10 (PubMed:24845384). The phosphorylated form (at Tyr-530) interacts with GATA3 (PubMed:15662016).
Post-translational Modifications: Phosphorylations at Ser-53, Tyr-77, Ser-225 and Ser-513 are regulated by mTORC1. Phosphorylation at Tyr-530 is essential for its interaction GATA3. Phosphorylation at Tyr-220, Tyr-266 and Tyr-305 enhances its transcriptional activator activity. Phosphorylation at Thr-303 is required for its interaction with NFATC2. Ubiquitinated at Lys-314, leading to its degradation by the proteasome. Ubiquitination is essential for controlling protein stability, binding to the T-box-binding element of the IFN-gamma promoter, and for interaction with NFATC2 through induction of phosphorylation at Thr-303 (By similarity). Deubiquitinated by USP10 leading to its stabilization (PubMed:24845384).

NCBI and Uniprot Product Information

• NCBI GI #: 7019549
• NCBI GeneID: 30009
• NCBI Accession #: NP_037483.1
• NCBI GenBank Nucleotide #: NM_013351.1
• UniProt Accession #: Q9UL17
• Molecular Weight: Observed: 58 kDa; Predicted: 59 kDa
• NCBI Official Full Name: T-box transcription factor TBX21
• NCBI Official Synonym Full Names: T-box 21
• NCBI Official Symbol: TBX21
• NCBI Official Synonym Symbols: TBET; T-PET; T-bet; TBLYM
• NCBI Protein Information: T-box transcription factor TBX21
• UniProt Protein Name: T-box transcription factor TBX21
• UniProt Gene Name: TBX21
• UniProt Synonym Gene Names: TBET; TBLYM; T-box protein 21