Rabbit anti-Human, Mouse TBX21 / T-bet Polyclonal Antibody | anti-TBX21 antibody

Rabbit Polyclonal (IgG) to Human TBX21 / T-bet

Cat. Num. AAA52435

• Gene Names: TBX21; TBET; T-PET; T-bet; TBLYM
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Peptide Column Affinity Chromatography
• Synonyms: TBX21 / T-bet, Antibody; Rabbit Polyclonal (IgG) to Human TBX21 / T-bet; Anti-TBX21 / T-bet Antibody (C-Terminus) IHC-plus; TBX21; T-bet; TBET; T-box 21; T-box protein 21; TBLYM; Transcription factor TBLYM; T-box expressed in T cells; T-PET; Human TBX21; anti-TBX21 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TBX21 antibody is human and mouse reactive.
• Purity/Purification: Peptide Column Affinity Chromatography
• Form/Format: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 535

• Applicable Applications for anti-TBX21 antibody: ELISA, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Immunogen Type: Synthetic peptide
• Immunogen Species: Human
• Immunogen Description: TBX21 / T-bet antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TBX2. The immunogen is located within the last 50 amino acids of TBX21.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.

WB (Western Blot)

(Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at 1 ug/ml.)

IF (Immunofluorescence)

(Immunofluorescence of TBX21 in 293 cells with TBX21 antibody at 5 ug/ml.)

IHC (Immunohistochemistry)

(Anti-TBX21 / T-bet antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 7019549
• NCBI GeneID: 30009
• NCBI Accession #: NP_037483.1
• NCBI GenBank Nucleotide #: NM_013351.1
• UniProt Accession #: Q9UL17
• NCBI Official Full Name: T-box transcription factor TBX21
• NCBI Official Synonym Full Names: T-box 21
• NCBI Official Symbol: TBX21
• NCBI Official Synonym Symbols: TBET; T-PET; T-bet; TBLYM
• NCBI Protein Information: T-box transcription factor TBX21; T-box protein 21; T-box expressed in T cells; transcription factor TBLYM; T-cell-specific T-box transcription factor T-bet
• UniProt Protein Name: T-box transcription factor TBX21
• UniProt Gene Name: TBX21
• UniProt Synonym Gene Names: TBET; TBLYM; T-box protein 21
• UniProt Entry Name: TBX21_HUMAN