Rabbit anti-Human TBX5 Polyclonal Antibody | anti-TBX5 antibody

TBX5 Antibody (N-term)

Cat. Num. AAA286901

• Gene Names: TBX5; HOS
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TBX5, Antibody; TBX5 Antibody (N-term); T-box transcription factor TBX5; T-box protein 5; TBX5; anti-TBX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TBX5.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 1-30
• Sequence Length: 518

• Applicable Applications for anti-TBX5 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Function: DNA-binding protein that regulates the transcription of several genes and is involved in heart development and limb pattern formation (PubMed:25725155, PubMed:25963046, PubMed:29174768, PubMed:26917986, PubMed:27035640, PubMed:8988164). Binds to the core DNA motif of NPPA promoter (PubMed:26926761).
• Cellular Location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201, ECO:0000269|PubMed:29174768}. Cytoplasm Note=Shuttles between the cytoplasm and the nucleus. Acetylation at Lys-339 promotes nuclear retention.
• Preparation and Storage: Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(TBX5 Antibody (N-term) (AAA286901)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TBX5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(TBX5 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the TBX5 antibody detected the TBX5 protein (arrow).)

WB (Western Blot)

(All lanes : Anti-TBX5 Antibody (N-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: HL-60 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-TBX5 antibody

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene.

Product Categories/Family for anti-TBX5 antibody

Cardiovascular

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Stirnimann, C.U., et al. J. Mol. Biol. 400(1):71-81(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Hong, K.W., et al. J. Hum. Genet. 55(6):336-341(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 18201892
• NCBI GeneID: 6910
• NCBI Accession #: NP_000183.2; NP_542448.1; NP_852259.1
• NCBI GenBank Nucleotide #: NM_000192.3
• UniProt Accession #: Q99593; O15301; Q96TB0; Q9Y4I2; A6ND77
• Molecular Weight: 57711
• NCBI Official Full Name: T-box transcription factor TBX5 isoform 1
• NCBI Official Synonym Full Names: T-box 5
• NCBI Official Symbol: TBX5
• NCBI Official Synonym Symbols: HOS
• NCBI Protein Information: T-box transcription factor TBX5
• UniProt Protein Name: T-box transcription factor TBX5
• UniProt Gene Name: TBX5
• UniProt Synonym Gene Names: T-box protein 5
• UniProt Entry Name: TBX5_HUMAN