IF (Immunofluorescence)
(AAA329046 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329046) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Rabbit TCF21 Polyclonal Antibody | anti-TCF21 antibody
TCF21 Antibody
Gene Names
TCF21; POD1; bHLHa23
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Synonyms
TCF21, Antibody; TCF21 Antibody; bHLHa23; Capsulin; Class A basic helix-loop-helix protein 23; Epicardin; POD 1; Pod-1; POD1; Podocyte expressed 1; Podocyte-expressed 1; TCF-21; tcf21; TCF21_HUMAN; Transcription factor 21; anti-TCF21 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TCF21 Antibody detects endogenous levels of total TCF21.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-TCF21 antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TCF21, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C.
Stable for 12 months from date of receipt.
Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA329046 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329046) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(AAA329046 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329046) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(Western blot analysis of extracts from EC304 cells, using TCF21 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 37 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from EC304 cells, using TCF21 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 37 kDa.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19kDa, 37kDa
NCBI Official Full Name
transcription factor 21
NCBI Official Synonym Full Names
transcription factor 21
NCBI Official Symbol
TCF21
NCBI Official Synonym Symbols
POD1; bHLHa23
NCBI Protein Information
transcription factor 21
UniProt Protein Name
Transcription factor 21
UniProt Gene Name
TCF21
UniProt Synonym Gene Names
BHLHA23; POD1
UniProt Entry Name
TCF21_HUMAN
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Product Notes
The TCF21 tcf21 (Catalog #AAA329046) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF21 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TCF21 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the TCF21 tcf21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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