FCM/FACS (Flow Cytometry)
(TCF21 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit TCF21 Polyclonal Antibody | anti-TCF21 antibody
TCF21 Antibody (C-term)
Gene Names
TCF21; POD1; bHLHa23
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TCF21, Antibody; TCF21 Antibody (C-term); Transcription factor 21; TCF-21; Capsulin; Class A basic helix-loop-helix protein 23; bHLHa23; Epicardin; Podocyte-expressed 1; Pod-1; TCF21; BHLHA23; POD1; anti-TCF21 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TCF21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the C-terminal region of human TCF21.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.32 (varies by lot)
Sequence Positions
144-173
Sequence Length
179
Applicable Applications for anti-TCF21 antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(TCF21 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(TCF21 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(TCF21 Antibody (C-term) western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).This demonstrates the TCF21 antibody detected the TCF21 protein (arrow).)
WB (Western Blot)
(TCF21 Antibody (C-term) western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).This demonstrates the TCF21 antibody detected the TCF21 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody (C-term). AAA286704 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody (C-term). AAA286704 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-TCF21 antibody
TCF21 encodes a transcription factor of the basic
helix-loop-helix family. The TCF21 product is mesoderm specific,
and expressed in embryonic epicardium, mesenchyme-derived tissues
of lung, gut, gonad, and both mesenchymal and glomerular epithelial
cells in the kidney. Two transcript variants encoding the same
protein have been found for this gene.
helix-loop-helix family. The TCF21 product is mesoderm specific,
and expressed in embryonic epicardium, mesenchyme-derived tissues
of lung, gut, gonad, and both mesenchymal and glomerular epithelial
cells in the kidney. Two transcript variants encoding the same
protein have been found for this gene.
Product Categories/Family for anti-TCF21 antibody
References
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)
Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008) :
Smith, L.T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(4):982-987(2006)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)
Melzer, D., et al. PLoS Genet. 4 (5), E1000072 (2008) :
Smith, L.T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(4):982-987(2006)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19715
NCBI Official Full Name
transcription factor 21
NCBI Official Synonym Full Names
transcription factor 21
NCBI Official Symbol
TCF21
NCBI Official Synonym Symbols
POD1; bHLHa23
NCBI Protein Information
transcription factor 21
UniProt Protein Name
Transcription factor 21
UniProt Gene Name
TCF21
UniProt Synonym Gene Names
BHLHA23; POD1; TCF-21; bHLHa23; Pod-1
UniProt Entry Name
TCF21_HUMAN
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Product Notes
The TCF21 tcf21 (Catalog #AAA286704) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 144-173. The TCF21 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's TCF21 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TCF21 tcf21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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