WB (Western Blot)
(Western blot of TCF7L1 / TCF3 antibody. Nuclear extract of PANC-1 cells (20 ?g) probed with TCF7L1 / TCF3 antibody (1:500).)
Rabbit anti-Human TCF7L1/TCF3 Polyclonal Antibody | anti-TCF7L1/TCF3 antibody
TCF7L1/TCF3 antibody (pAb)
Gene Names
TCF7L1; TCF3; TCF-3
Reactivity
Human
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
TCF7L1/TCF3, Antibody; TCF7L1/TCF3 antibody (pAb); anti-TCF7L1/TCF3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-TCF7L1/TCF3 antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This TCF7L1/TCF3 antibody was raised against a peptide from the N-terminus of human TCF7L1/TCF3.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Western blot of TCF7L1 / TCF3 antibody. Nuclear extract of PANC-1 cells (20 ?g) probed with TCF7L1 / TCF3 antibody (1:500).)
WB (Western Blot)
(Western blot of TCF7L1 / TCF3 antibody. Nuclear extract of PANC-1 cells (20 ?g) probed with TCF7L1 / TCF3 antibody (1:500).)
ChIP (Chromatin Immunoprecipitation)
(TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from undifferentiated hESC cells and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 26 million sequence tags were mapped to identify TCF7L1 / TCF3 binding sites. The image shows binding across a region of chromosome 17. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
ChIP (Chromatin Immunoprecipitation)
(TCF7L1 / TCF3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from undifferentiated hESC cells and 7 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 26 million sequence tags were mapped to identify TCF7L1 / TCF3 binding sites. The image shows binding across a region of chromosome 17. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Related Product Information for anti-TCF7L1/TCF3 antibody
Short Description: Our TCF7L1/TCF3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human samples.
Background: TCF3/TCF7L1 (T-cell factor 3, TCF7L1) is a member of the TCF/LEF family and a component of the Wnt signaling pathway and a dominant downstream effector in embryonic stem cells (ESCs). TCF3 binds to DNA and serves as both a repressor as well as an activator of transcription. TCF3 brings developmental signals directly to the core regulatory circuitry of ES cells to influence the balance between pluripotency and differentiation. TCF3 transcriptionally represses many genes important for maintaining pluripotency and self-renewal, as well as those involved in lineage commitment and stem cell differentiation. This effect is in part mediated by the corepressors transducin-like enhancer of split 2 (TLE2) and C-terminal Binding Protein (CtBP).
Background: TCF3/TCF7L1 (T-cell factor 3, TCF7L1) is a member of the TCF/LEF family and a component of the Wnt signaling pathway and a dominant downstream effector in embryonic stem cells (ESCs). TCF3 binds to DNA and serves as both a repressor as well as an activator of transcription. TCF3 brings developmental signals directly to the core regulatory circuitry of ES cells to influence the balance between pluripotency and differentiation. TCF3 transcriptionally represses many genes important for maintaining pluripotency and self-renewal, as well as those involved in lineage commitment and stem cell differentiation. This effect is in part mediated by the corepressors transducin-like enhancer of split 2 (TLE2) and C-terminal Binding Protein (CtBP).
Product Categories/Family for anti-TCF7L1/TCF3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
64kDa
NCBI Official Full Name
transcription factor 7-like 1
NCBI Official Synonym Full Names
transcription factor 7 like 1
NCBI Official Symbol
TCF7L1
NCBI Official Synonym Symbols
TCF3; TCF-3
NCBI Protein Information
transcription factor 7-like 1
UniProt Protein Name
Transcription factor 7-like 1
UniProt Gene Name
TCF7L1
UniProt Synonym Gene Names
TCF3; TCF-3
UniProt Entry Name
TF7L1_HUMAN
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Product Notes
The TCF7L1/TCF3 tcf7l1 (Catalog #AAA59982) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TCF7L1/TCF3 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCF7L1/TCF3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the TCF7L1/TCF3 tcf7l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF7L1/TCF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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