IHC (Immunohistochemisry)
(AAA328913 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit TDP1 Polyclonal Antibody | anti-TDP1 antibody
TDP1 Antibody
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Zebrafish(83%), Horse(100%), Rabbit(100%), Dog(88%), Xenopus(88%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TDP1, Antibody; TDP1 Antibody; AI838772; AW493413; FLJ11090; MGC104252; MGC112732; RP24-311F12.2; SCAN1; TDP1; TYDP; TYDP1_HUMAN; Tyr-DNA phosphodiesterase 1; Tyrosyl-DNA phosphodiesterase 1; anti-TDP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(83%), Horse(100%), Rabbit(100%), Dog(88%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Zebrafish(83%), Horse(100%), Rabbit(100%), Dog(88%), Xenopus(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
TDP1 Antibody detects endogenous levels of total TDP1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
608
Applicable Applications for anti-TDP1 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human TDP1, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemisry)
(AAA328913 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA328913 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328913 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328913 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using TDP1 Antibody.Lane 1: B16F10 cells, treated with blocking peptide;Lane 2: B16F10 cells;Lane 3: HepG2 cells.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using TDP1 Antibody.Lane 1: B16F10 cells, treated with blocking peptide;Lane 2: B16F10 cells;Lane 3: HepG2 cells.)
Related Product Information for anti-TDP1 antibody
Function: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.
Post Translational Modifications: Phosphorylated on serine and/or threonine residues, but not on tyrosine residues.
Subcellular Location: Nucleus. Cytoplasm.
Tissue Specificity: Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
Subunit Structure: Monomer.
Similarity: Belongs to the tyrosyl-DNA phosphodiesterase family.
Post Translational Modifications: Phosphorylated on serine and/or threonine residues, but not on tyrosine residues.
Subcellular Location: Nucleus. Cytoplasm.
Tissue Specificity: Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus.
Subunit Structure: Monomer.
Similarity: Belongs to the tyrosyl-DNA phosphodiesterase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)68kD.
Predicted Molecular Weight: (Calculated)68kDa.
Predicted Molecular Weight: (Calculated)68kDa.
NCBI Official Full Name
Tyrosyl-DNA phosphodiesterase 1
NCBI Official Synonym Full Names
tyrosyl-DNA phosphodiesterase 1
NCBI Official Symbol
TDP1
NCBI Protein Information
tyrosyl-DNA phosphodiesterase 1
UniProt Protein Name
Tyrosyl-DNA phosphodiesterase 1
UniProt Gene Name
TDP1
UniProt Synonym Gene Names
Tyr-DNA phosphodiesterase 1
UniProt Entry Name
TYDP1_HUMAN
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Product Notes
The TDP1 tdp1 (Catalog #AAA328913) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TDP1 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Zebrafish(83%), Horse(100%), Rabbit(100%), Dog(88%), Xenopus(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's TDP1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TDP1 tdp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TDP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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