Rabbit anti-Human TFEB Polyclonal Antibody | anti-TFEB antibody

Rabbit anti-TFEB Antibody, Affinity Purified

Cat. Num. AAA212255

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: TFEB, Antibody; Rabbit anti-TFEB Antibody, Affinity Purified; ALPHATFEB; transcription factor EB; TCFEB; BHLHE35; class E basic helix-loop-helix protein 35; T-cell transcription factor EB; Transcription factor EB; anti-TFEB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-TFEB antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 350 and 400
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human TFEB by western blot. Samples:Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody AAA212255 (lot AAA212255-3) used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 10 second.)

IP (Immunoprecipitation)

(Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody AAA212255 (lot AAA212255-3) used for IP at 6 ug per reaction. TFEB was also immunoprecipitated by rabbit anti-TFEB antibody and rabbit anti-TFEB recombinant monoclonal antibody . For blotting immunoprecipitated TFEB, was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)

Related Product Information for anti-TFEB antibody

Transcription factor EB (TFEB) specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, TFEB activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. TFEB specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression; thus, TFEB plays a central role in expression of lysosomal genes. TFEB also specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P19484].

Product Categories/Family for anti-TFEB antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 268607698
• NCBI GeneID: 7942
• NCBI Accession #: NP_009093.1
• NCBI GenBank Nucleotide #: NM_007162
• UniProt Accession #: P19484; Q709B3; Q7Z6P9; Q9BRJ5; Q9UJD8
• NCBI Official Full Name: Homo sapiens transcription factor EB (TFEB), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
• UniProt Protein Name: Transcription factor EB
• UniProt Gene Name: TFEB
• UniProt Synonym Gene Names: BHLHE35; bHLHe35
• UniProt Entry Name: TFEB_HUMAN