Rabbit anti-Mouse, Human Tgfb1 Polyclonal Antibody | anti-Tgfb1 antibody

Tgfb1 Antibody

Cat. Num. AAA232705

• Gene Names: Tgfb1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
• Reactivity: Mouse, Human
• Applications: Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: Tgfb1, Antibody; Tgfb1 Antibody; Transforming growth factor beta-1TGF-beta-1; Tgfb1; anti-Tgfb1 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 390

• Applicable Applications for anti-Tgfb1 antibody: WB (Western Blot), ELISA
• Immunogen: Recombinant Mouse Transforming growth factor beta-1 protein (30-391AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IP (Immunoprecipitation)

(Immunoprecipitating Tgfb1 in MCF-7 whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA232705 in MCF-7 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA232705 (8ug)+ MCF-7 whole cell lysate (500ug)Lane 3: MCF-7 whole cell lysate (10ug))

WB (Western Blot)

(Western BlotPositive WB detected in:MCF-7 whole cell lysateAll lanes: Tgfb1 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 45 kDaObserved band size: 45 kDa)

Related Product Information for anti-Tgfb1 antibody

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (PubMed: 18368049). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 6755775
• NCBI GeneID: 21803
• NCBI Accession #: NP_035707.1
• NCBI GenBank Nucleotide #: NM_011577.2
• UniProt Accession #: P04202
• Molecular Weight: 44,310 Da
• NCBI Official Full Name: transforming growth factor beta-1 preproprotein
• NCBI Official Synonym Full Names: transforming growth factor, beta 1
• NCBI Official Symbol: Tgfb1
• NCBI Official Synonym Symbols: Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
• NCBI Protein Information: transforming growth factor beta-1
• UniProt Protein Name: Transforming growth factor beta-1
• UniProt Gene Name: Tgfb1
• UniProt Synonym Gene Names: TGF-beta-1; LAP
• UniProt Entry Name: TGFB1_MOUSE