Standard Curve (Sample)
(Human LAP(TGF-beta1) PicoKine ELISA Kit standard curve)
Human LAP(TGF-beta1) ELISA Kit | TGFB1 elisa kit
Human LAP(TGF-beta1) PicoKine ELISA Kit
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Reactivity
Human
Synonyms
LAP(TGF-beta1); N/A; Human LAP(TGF-beta1) PicoKine ELISA Kit; Transforming growth factor beta-1; transforming growth factor, beta 1; Camurati-Engelmann disease; CED; DPD1; LAP; Latency-associated peptide; TGF beta 1 protein; TGF-beta-1; TGFB; tgfb1; TGFB1_HUMAN; TGFbeta; Transforming growth factor beta 1; TGFB1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human LAP(TGF-beta1)
Sequence Length
390
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human LAP(TGF-beta1) PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human LAP(TGF-beta1) PicoKine ELISA Kit standard curve)
Related Product Information for TGFB1 elisa kit
Principle of the assay: human LAP(TGF-beta1) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for LAP(TGF-beta1) has been precoated onto 96-well plates. Standards(sf21, L30-R278) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LAP(TGF-beta1) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LAP(TGF-beta1) amount of sample captured in plate.
Background: TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells.
Background: TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. Processing and cleavage of the precursor protein between amino acids 278 and 279 results in the formation of LAP dimers and TGF beta dimers that then non-covalently associate with each other to form the small latent TGF beta complex. LAP is secreted and can be found in the extracellular matrix. In addition, LAP can also be expressed on platelets and activated regulatory T cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,341 Da
NCBI Official Full Name
Transforming growth factor beta-1
NCBI Official Synonym Full Names
transforming growth factor, beta 1
NCBI Official Symbol
TGFB1
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
NCBI Protein Information
transforming growth factor beta-1; TGF-beta-1; latency-associated peptide; prepro-transforming growth factor beta-1
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1
UniProt Synonym Gene Names
TGFB; TGF-beta-1; LAP
UniProt Entry Name
TGFB1_HUMAN
Similar Products
Product Notes
The Human TGFB1 tgfb1 (Catalog #AAA45915) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45915 ELISA Kit recognizes Human TGFB1. It is sometimes possible for the material contained within the vial of "LAP(TGF-beta1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.