WB (Western Blot)
(Western blot analysis of Hela cell lysate, using TGFBI Antibody. The lane on the left is treated with the antigen-specific peptide.)
Rabbit TGFBI Polyclonal Antibody | anti-TGFBI antibody
TGFBI Antibody
Gene Names
TGFBI; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TGFBI, Antibody; TGFBI Antibody; RGD containing collagen associated protein; AI181842; AI747162; Beta ig; Beta ig h3; Beta ig-h3; BGH3_HUMAN; Big h3; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; Kerato epithelin; Kerato-epithelin; LCD1; MGC150270; RGD CAP; RGD-CAP; RGD-containing collagen-associated protein; TGFBI; TGFBI transforming growth factor, beta induced, 68kDa; Transforming growth factor beta induced protein ig h3; Transforming growth factor-beta-induced protein ig-h3; anti-TGFBI antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TGFBI antibody detects endogenous levels of total TGFBI
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
683
Applicable Applications for anti-TGFBI antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide of human TGFBI
Subcellular Location
Secreted > Extracellular Space > Extracellular Matrix. May be associated both with microfibrils and with the cell surface.
Tissue Specificity
Highly expressed in the corneal epithelium (PubMed:27609313, PubMed:8077289). Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas (PubMed:8077289).
Predicted Cross Reactivity
Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Bovine (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Hela cell lysate, using TGFBI Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Hela cell lysate, using TGFBI Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry)
(AAA326764 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA326764 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-TGFBI antibody
Description: TGFBI is a RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. TGFBI plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. TGFBI is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Function: Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity).
Subunit Structure: Binds to type I, II, and IV collagens.
Post-translational Modifications: Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; these residues may be required for binding to calcium (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833). The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain.
Function: Plays a role in cell adhesion (PubMed:8024701). May play a role in cell-collagen interactions (By similarity).
Subunit Structure: Binds to type I, II, and IV collagens.
Post-translational Modifications: Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; these residues may be required for binding to calcium (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833). The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 68 kDa
Predicted: 75 kDa
Predicted: 75 kDa
NCBI Official Full Name
transforming growth factor-beta-induced protein ig-h3
NCBI Official Synonym Full Names
transforming growth factor beta induced
NCBI Official Symbol
TGFBI
NCBI Official Synonym Symbols
CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1
NCBI Protein Information
transforming growth factor-beta-induced protein ig-h3
UniProt Protein Name
Transforming growth factor-beta-induced protein ig-h3
UniProt Gene Name
TGFBI
UniProt Synonym Gene Names
BIGH3; Beta ig-h3; RGD-CAP
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Product Notes
The TGFBI tgfbi (Catalog #AAA326764) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TGFBI Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TGFBI can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TGFBI tgfbi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGFBI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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