IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with Rabbit anti-TGFBR1)(pSer165) antibody (Cat#AAA71346) at 1:50 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rabbit TGFBR1 Polyclonal Antibody | anti-TGFBR1 antibody
Rabbit anti TGFBR1 (pS165) Polyclonal Antibody
Gene Names
TGFBR1; AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; tbetaR-I
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
The Rabbit IgG is purified by Site-modified Epitope Affinity Purification
Synonyms
TGFBR1, Antibody; Rabbit anti TGFBR1 (pS165) Polyclonal Antibody; Transforming Growth Factor beta Receptor Type 1 (TGFBR1); ALK5; anti-TGFBR1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes human protein. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-modified Epitope Affinity Purification
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Sequence Length
507
Applicable Applications for anti-TGFBR1 antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
Antigen Preparation
A synthetic peptide derived from internal sequence (-EEDP-*S-LDRP) with a phosphorylation site at Serine 165 of mouse TGFbeta receptor type-1. This sequence is also identical within human, rat, mouse, chicken, bovine and dog species
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at –20°C to –70°C. The antibodies can be stored at 2°C - 8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with Rabbit anti-TGFBR1)(pSer165) antibody (Cat#AAA71346) at 1:50 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
IHC (Immunohiostchemistry)
(Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with Rabbit anti-TGFBR1)(pSer165) antibody (Cat#AAA71346) at 1:50 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
WB (Western Blot)
(The cell lysate derived from TGF beta peptide (10 ng/ml for 30 minutes) stimulated MCF-7 cell lysate was immunoprobed by Rabbit anti-TGF?R1 (Cat#AAA71346) at 1:500. An immunoreactive band is observed around ~55 kDa)
WB (Western Blot)
(The cell lysate derived from TGF beta peptide (10 ng/ml for 30 minutes) stimulated MCF-7 cell lysate was immunoprobed by Rabbit anti-TGF?R1 (Cat#AAA71346) at 1:500. An immunoreactive band is observed around ~55 kDa)
Related Product Information for anti-TGFBR1 antibody
Transforming growth factor ? isoforms (TGF-?1, 2, 3) play vital roles in regulating cellular growth and differentiation, and they signal through a highly restricted subset of receptors known as TGF-? type I receptor (T?R-I) and TGF-? type II receptor (T?R-II). Expression of T beta R-I is more ubiquitous than that of T beta R-II. There was no staining of T beta R-II in the central nervous system, while intense T beta R-I staining was found specifically in nervous tissues. Expression of T beta R-I and T beta R-II was mostly coincident with that of their ligands, suggesting that TGF-beta s act as multiple mediators during organogenesis. TGF-? binds with high affinity to its receptor, a transmembrane protein with a cytoplasmic serine/threonine kinase domain, to form a dynamic dimer with phosphorylation in the cytoplasmic Ser/Thr residuals which cause its kinase activity and association with another TGF beta-binding protein, the type I receptor, to signal growth inhibition and early gene responses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55.0
NCBI Official Full Name
TGF-beta receptor type-1 isoform 3
NCBI Official Synonym Full Names
transforming growth factor beta receptor 1
NCBI Official Symbol
TGFBR1
NCBI Official Synonym Symbols
AAT5; ALK5; ESS1; LDS1; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4; tbetaR-I
NCBI Protein Information
TGF-beta receptor type-1
UniProt Protein Name
TGF-beta receptor type-1
UniProt Gene Name
TGFBR1
UniProt Synonym Gene Names
ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I
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Product Notes
The TGFBR1 tgfbr1 (Catalog #AAA71346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti TGFBR1 (pS165) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TGFBR1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the TGFBR1 tgfbr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGFBR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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