IHC (Immunohistochemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (THRB1) Polyclonal Antibody, Unconjugated (bs-11440R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Rabbit anti-Rat, Cow THRB1 Polyclonal Antibody | anti-THRB1 antibody
THRB1 Polyclonal Antibody
IHC (Immunohistochemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (THRB1) Polyclonal Antibody, Unconjugated (bs-11440R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (THRB1) Polyclonal Antibody, Unconjugated (bs-11440R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistchemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (THRB1) Polyclonal Antibody, Unconjugated (bs-11440R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistchemistry-Paraffin)
(Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (THRB1) Polyclonal Antibody, Unconjugated (bs-11440R) at 1:200 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
WB (Western Blot)
(Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Liver (Rat) Lysate at 40 ug Lane 3: K562 (Human) Cell Lysate at 30 ug Lane 4: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti- THRB1 (bs-11440R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53/46 kD Observed band size: 53/46 kD)
WB (Western Blot)
(Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Liver (Rat) Lysate at 40 ug Lane 3: K562 (Human) Cell Lysate at 30 ug Lane 4: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti- THRB1 (bs-11440R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53/46 kD Observed band size: 53/46 kD)
FCM (Flow Cytometry)
(Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM (Flow Cytometry)
(Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
WB (Western Blot)
(Dilution: Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Pituitary (Mouse) Lysate at 40 ug Lane 3: Adrenal gland (Mouse) Lysate at 40 ug Lane 4: Muscle (Mouse) Lysate at 40 ug Lane 5: Cerebrum (Rat) Lysate at 40 ug Lane 6: Pituitary (Rat) Lysate at 40 ug Lane 7: Adrenal gland (Rat) Lysate at 40 ug Lane 8: Muscle (Rat) Lysate at 40 ug Lane 9: K562 (Human) Cell Lysate at 30 ug Lane 10: Siha (Human) Cell Lysate at 30 ug Lane 11: A431 (Human) Cell Lysate at 30 ug Lane 12: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-THRB1 (bs-11440R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53/46 kD Observed band size: 53/46 kD)
WB (Western Blot)
(Dilution: Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Pituitary (Mouse) Lysate at 40 ug Lane 3: Adrenal gland (Mouse) Lysate at 40 ug Lane 4: Muscle (Mouse) Lysate at 40 ug Lane 5: Cerebrum (Rat) Lysate at 40 ug Lane 6: Pituitary (Rat) Lysate at 40 ug Lane 7: Adrenal gland (Rat) Lysate at 40 ug Lane 8: Muscle (Rat) Lysate at 40 ug Lane 9: K562 (Human) Cell Lysate at 30 ug Lane 10: Siha (Human) Cell Lysate at 30 ug Lane 11: A431 (Human) Cell Lysate at 30 ug Lane 12: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-THRB1 (bs-11440R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53/46 kD Observed band size: 53/46 kD)
FCM (Flow Cytometry)
(Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM (Flow Cytometry)
(Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM (Flow Cytometry)
(Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
FCM (Flow Cytometry)
(Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-THRB1 antibody (bs-11440R) Dilution: 1 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1 µg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20 degree C. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.)
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Product Notes
The THRB1 (Catalog #AAA28811) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The THRB1 Polyclonal Antibody reacts with Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's THRB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunofluorescence (IF), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the THRB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "THRB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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