Rabbit anti-Human, mouse TIE Polyclonal Antibody | anti-TIE1 antibody

TIE Antibody (N-term)

Cat. Num. AAA283671

• Gene Names: TIE1; TIE; JTK14
• Reactivity: Human, mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TIE, Antibody; TIE Antibody (N-term); Tyrosine-protein kinase receptor Tie-1; TIE1; TIE; anti-TIE1 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TIE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human TIE.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 1.22 (varies by lot)
• Sequence Positions: 12-42
• Sequence Length: 1138

• Applicable Applications for anti-TIE1 antibody: IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with TIE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of anti-TIE Antibody (N-term) in mouse bladder tissue lysates (35ug/lane). TIE(arrow) was detected using the purified Pab.)

Related Product Information for anti-TIE1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Product Categories/Family for anti-TIE1 antibody

Cancer; Cardiovascular; Developmental Biology; Metabolism; Signal Transduction

References

Verstovsek, S., et al., Cancer 94(5):1517-1521 (2002).
Tsiamis, A.C., et al., Microvasc. Res. 63(2):149-158 (2002).
Sato, T.N., et al., Nature 376(6535):70-74 (1995).
Partanen, J., et al., Mol. Cell. Biol. 12(4):1698-1707 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4885631
• NCBI GeneID: 7075
• NCBI Accession #: NP_005415.1
• NCBI GenBank Nucleotide #: NM_005424.4
• UniProt Accession #: P35590; B5A949; B5A950
• Molecular Weight: 125090
• NCBI Official Full Name: tyrosine-protein kinase receptor Tie-1 isoform 1
• NCBI Official Synonym Full Names: tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• NCBI Official Symbol: TIE1
• NCBI Official Synonym Symbols: TIE; JTK14
• NCBI Protein Information: tyrosine-protein kinase receptor Tie-1
• UniProt Protein Name: Tyrosine-protein kinase receptor Tie-1
• UniProt Gene Name: TIE1
• UniProt Synonym Gene Names: TIE
• UniProt Entry Name: TIE1_HUMAN