IP (Immunoprecipitation)
(Detection of human TIGAR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TIGAR antibody AAA211834 used for IP at 10 ug/mg lysate. TIGAR was less efficiently immunoprecipitated by rabbit anti-TIGAR antibody which recognizes an upstream epitope. For blotting immunoprecipitated TIGAR, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human TIGAR Polyclonal Antibody | anti-TIGAR antibody
Rabbit anti-TIGAR Antibody, Affinity Purified
Gene Names
C12orf5; FR2BP; TIGAR
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
TIGAR, Antibody; Rabbit anti-TIGAR Antibody, Affinity Purified; TP53-induced glycolysis and apoptosis regulator; TP53-induced glycolysis regulatory phosphatase; probable fructose-2,6-bisphosphatase TIGAR; fructose-2,6-bisphosphate 2-phosphatase; fructose-2,6-bisphosphatase TIGAR; FR2BP; C12orf5; transactivated by NS3TP2 protein; Fructose-2,6-bisphosphatase TIGAR; TP53 induced glycolysis regulatory phosphatase; anti-TIGAR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TIGAR antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 220 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human TIGAR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TIGAR antibody AAA211834 used for IP at 10 ug/mg lysate. TIGAR was less efficiently immunoprecipitated by rabbit anti-TIGAR antibody which recognizes an upstream epitope. For blotting immunoprecipitated TIGAR, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human TIGAR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TIGAR antibody AAA211834 used for IP at 10 ug/mg lysate. TIGAR was less efficiently immunoprecipitated by rabbit anti-TIGAR antibody which recognizes an upstream epitope. For blotting immunoprecipitated TIGAR, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human TIGAR by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TIGAR (Cat. No. AAA211834 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human TIGAR by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-TIGAR (Cat. No. AAA211834 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-TIGAR antibody
TIGAR (TP53-induced glycolysis and apoptosis regulator) is a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis [taken from NCBI GeneID:57103].
Product Categories/Family for anti-TIGAR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,063 Da
NCBI Official Full Name
fructose-2,6-bisphosphatase TIGAR
NCBI Official Synonym Full Names
chromosome 12 open reading frame 5
NCBI Official Symbol
C12orf5
NCBI Official Synonym Symbols
FR2BP; TIGAR
NCBI Protein Information
fructose-2,6-bisphosphatase TIGAR; TP53-induced glycolysis and apoptosis regulator; fructose-2,6-bisphosphate 2-phosphatase; probable fructose-2,6-bisphosphatase TIGAR; transactivated by NS3TP2 protein
UniProt Protein Name
Fructose-2,6-bisphosphatase TIGAR
UniProt Gene Name
TIGAR
UniProt Synonym Gene Names
C12orf5
UniProt Entry Name
TIGAR_HUMAN
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Product Notes
The TIGAR tigar (Catalog #AAA211834) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TIGAR Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIGAR can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the TIGAR tigar for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIGAR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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