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IHC (Immunohistchemistry)
(Figure 5 Immunohistochemistry Validation of TIM-1Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Rabbit anti-Human, Mouse TIM-1 Polyclonal Antibody | anti-HAVCR1 antibody
TIM-1 Antibody
Gene Names
HAVCR1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
TIM-1 Antibody is affinity chromatography purified via peptide column.
Synonyms
TIM-1; Polyclonal Antibody; TIM-1 Antibody; TIM; KIM1; TIM1; HAVCR; KIM-1; TIMD1; TIMD-1; HAVCR-1; Hepatitis A virus cellular receptor 1; Kidney injury molecule 1; HAVcr-1; hepatitis A virus cellular receptor 1; anti-HAVCR1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
TIM-1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
364
Applicable Applications for anti-HAVCR1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
TIM-1 antibody can be used for the detection of TIM-1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Knockdown (Knockout, KO validated) Antibody
Conjugate
Unconjugated
Immunogen
TIM-1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human TIM-1.
Buffer
TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
IHC (Immunohistchemistry)
(Figure 5 Immunohistochemistry Validation of TIM-1Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistchemistry)
(Figure 5 Immunohistochemistry Validation of TIM-1Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of TIM-1Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1 with 3809 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of TIM-1Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1 with 3809 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.)
WB (Western Blot)
(Figure 4 Western Blot Validation in the Mouse Cell LineLoading: 15 μg of NIH/3T3 cell lysates.Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 4 Western Blot Validation in the Mouse Cell LineLoading: 15 μg of NIH/3T3 cell lysates.Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 Validation with TIM-1 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2)Loading: 15 μg of HeLa whole cell lysates per lane.Antibodies: 3809 (8 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 Validation with TIM-1 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2)Loading: 15 μg of HeLa whole cell lysates per lane.Antibodies: 3809 (8 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Related Product Information for anti-HAVCR1 antibody
TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40 kDa
NCBI Official Full Name
hepatitis A virus cellular receptor 1
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 1
NCBI Official Symbol
HAVCR1
NCBI Official Synonym Symbols
TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1
NCBI Protein Information
hepatitis A virus cellular receptor 1; kidney injury molecule 1; T-cell membrane protein 1; T-cell immunoglobulin mucin receptor 1; T-cell immunoglobulin mucin family member 1; T cell immunoglobin domain and mucin domain protein 1
UniProt Protein Name
Hepatitis A virus cellular receptor 1
UniProt Gene Name
HAVCR1
UniProt Synonym Gene Names
KIM1; TIM1; TIMD1; HAVcr-1; KIM-1; TIMD-1; TIM; TIM-1
UniProt Entry Name
HAVR1_HUMAN
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Product Notes
The HAVCR1 havcr1 (Catalog #AAA10935) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIM-1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TIM-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). TIM-1 antibody can be used for the detection of TIM-1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Knockdown (Knockout, KO validated) Antibody. Researchers should empirically determine the suitability of the HAVCR1 havcr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIM-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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