IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug Timm29 antibody. Western blot was performed from the immunoprecipitate using Timm29 antibody at a dilution of 1:1000.)
Rabbit anti-Human, Mouse Timm29 Polyclonal Antibody | anti-Timm29 antibody
Timm29 Rabbit pAb
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Affinity purification
Synonyms
Timm29, Antibody; Timm29 Rabbit pAb; TIM29; 1810026J23Rik; anti-Timm29 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Sequence
MNNLLPRSVKMHIKYDLKGSTYKRRASQKEREKPLPTFKDLDFLQDIPDGLFLDADMYNALCKTLQRDCLVLQSFKIMDYSLLMSIHNIDHAQREPLSSETQYSVDTRRPAPQKALYSTAMESIQGEARRGGTMETDDHMGGIPARNSKGERLLLYIGIIDILQSYRFVKKLEHSWKALVHDGDTVSVHRPGFYAERFQRFMCNTVFKKIPCVHLGRPDVLPQTPPLEEISEGSPIPDPSFSPLVGETLQMLTTSTTLEKLEVAESEFTH
Applicable Applications for anti-Timm29 antibody
IP (Immunoprecipitation), WB (Western Blot)
Positive Samples
293T, A-549, PC-3, Mouse liver
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 157-266 of mouse Timm29 (NP_848734.1).
Cellular Location
mitochondrial inner membrane, mitochondrial intermembrane space
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug Timm29 antibody. Western blot was performed from the immunoprecipitate using Timm29 antibody at a dilution of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300ug extracts of A-549 cells using 3ug Timm29 antibody. Western blot was performed from the immunoprecipitate using Timm29 antibody at a dilution of 1:1000.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Timm29 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Timm29 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 120s.)
Related Product Information for anti-Timm29 antibody
Component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. The TIM22 complex forms a twin-pore translocase that uses the membrane potential as the external driving force. Required for the stability of the TIM22 complex and functions in the assembly of the TIMM22 protein into the TIM22 complex. May facilitate cooperation between TIM22 and TOM complexes by interacting with TOMM40.
Product Categories/Family for anti-Timm29 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,119 Da
NCBI Official Full Name
phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform 4
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
NCBI Official Symbol
PIP5K1A
NCBI Protein Information
phosphatidylinositol 4-phosphate 5-kinase type-1 alpha; 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha; 68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha; PIP5K1-alpha; PIP5KIalpha; phosphatidylinositol 4-phosphate 5-kinase type
UniProt Protein Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
UniProt Gene Name
PIP5K1A
UniProt Synonym Gene Names
PIP5K1-alpha; PtdIns(4)P-5-kinase 1 alpha; PIP5KIalpha
UniProt Entry Name
PI51A_HUMAN
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Product Notes
The Timm29 pip5k1a (Catalog #AAA282199) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Timm29 Rabbit pAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Timm29 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Timm29 pip5k1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MNNLLPRSVK MHIKYDLKGS TYKRRASQKE REKPLPTFKD LDFLQDIPDG LFLDADMYNA LCKTLQRDCL VLQSFKIMDY SLLMSIHNID HAQREPLSSE TQYSVDTRRP APQKALYSTA MESIQGEARR GGTMETDDHM GGIPARNSKG ERLLLYIGII DILQSYRFVK KLEHSWKALV HDGDTVSVHR PGFYAERFQR FMCNTVFKKI PCVHLGRPDV LPQTPPLEEI SEGSPIPDPS FSPLVGETLQ MLTTSTTLEK LEVAESEFTH. It is sometimes possible for the material contained within the vial of "Timm29, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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