<
WB (Western Blot)
(Detection of human Tip41 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, A-549, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (AAA211079 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse Tip41 Polyclonal Antibody | anti-Tip41 antibody
Rabbit anti-Tip41 Antibody, Affinity Purified
Gene Names
TIPRL; TIP; TIP41; TIPRL1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Tip41, Antibody; Rabbit anti-Tip41 Antibody, Affinity Purified; putative MAPK-activating protein PM10; TIP; TIP41; TIP41, TOR signaling pathway regulator-like; TIP41-like protein; TIPRL1; type 2A-interacting protein; TOR signaling pathway regulator; anti-Tip41 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-Tip41 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 222 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Tip41 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, A-549, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (AAA211079 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Tip41 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, A-549, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (AAA211079 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse Tip41 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (AAA211079 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of mouse Tip41 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Tip41 antibody (AAA211079 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human Tip41 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Tip41 antibody (AAA211079 lot 2) used for IP at 6 ug per reaction. Tip41 was also immunoprecipitated by a previous lot of this antibody (AAA211079 lot 1). For blotting immunoprecipitated Tip41, AAA211079 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Tip41 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Tip41 antibody (AAA211079 lot 2) used for IP at 6 ug per reaction. Tip41 was also immunoprecipitated by a previous lot of this antibody (AAA211079 lot 1). For blotting immunoprecipitated Tip41, AAA211079 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human Tip41 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Tip41 (AAA211079 Lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Tip41 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- Tip41 (AAA211079 Lot 2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-Tip41 antibody
In Saccharomyces cerevisiae Tip41 is implicated to play a role in the TOR (target of rapamycin) pathway which regulates cell growth in response to nutrient signals. Tip 41 negatively regulates the TOR pathway by binding and inhibiting TAP42, an inhibitor of the SIT4 phosphatase.
Product Categories/Family for anti-Tip41 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,214 Da
NCBI Official Full Name
TIP41-like protein isoform 1
NCBI Official Synonym Full Names
TOR signaling pathway regulator
NCBI Official Symbol
TIPRL
NCBI Official Synonym Symbols
TIP; TIP41; TIPRL1
NCBI Protein Information
TIP41-like protein
UniProt Protein Name
TIP41-like protein
UniProt Gene Name
TIPRL
UniProt Entry Name
TIPRL_HUMAN
Similar Products
Product Notes
The Tip41 tiprl (Catalog #AAA211079) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Tip41 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Tip41 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Tip41 tiprl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tip41, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.