Rabbit anti-Human TIPIN Polyclonal Antibody | anti-TIPIN antibody

Anti-TIPIN Antibody, Rabbit Polyclonal

Cat. Num. AAA254558

• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: TIPIN, Antibody; Anti-TIPIN Antibody, Rabbit Polyclonal; TIPIN Antibody, Rabbit PAb, Antigen Affinity Purified; TIMELESS interacting protein; anti-TIPIN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TIPIN
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-TIPIN antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human TIPIN
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TIPIN, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(TIPIN was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-TIPIN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TIPIN rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :53 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of TIPIN in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TIPIN polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.)

WB (Western Blot)

(Anti-TIPIN rabbit polyclonal antibody at 1:500 dilutionLane A: HCT116 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:50 kDa)

Related Product Information for anti-TIPIN antibody

Tipin was originally isolated as a protein interacting with Timeless/Tim1/Tim (Tim), which is known to be involved in both circadian rhythm and cell cycle checkpoint regulation. Tim and Tipin form a stable heterodimeric complex that influences checkpoint responses and replication fork progression. The endogenous Tim and Tipin proteins in human cells, interacting through the N-terminal segment of each molecule, form a complex throughout the cell cycle. Tipin and Tim are expressed in the interphase nuclei mostly at constant levels during the cell cycle, and small fractions are recovered in the chromatin-enriched fractions during the S phase. Depletion of endogenous Tipin results in a reduced growth rate, and this may be due in part to inefficient progression of S phase and DNA synthesis. Mammalian Tipin is a checkpoint mediator that cooperates with Tim and may regulate the nuclear relocation of Claspin in response to replication checkpoint.

References

Yoshizawa-Sugata N, et al. (2007) Human tim/timeless-interacting protein, tipin, is required for efficient progression of s phase and DNA replication checkpoint. J Biol Chem 282 (4): 2729-2740.Errico A, et al. (2009) Tipin/tim1/and1 protein complex promotes pol alpha chromatin binding and sister chromatid cohesion. Embo j 28 (23): 3681-3692.Chou DM, et al. (2006) Tipin and timeless form a mutually protective complex required for genotoxic stress resistance and checkpoint function. Proc Natl Acad Sci U S A 103 (48): 18143-18147.Cho WH, et al. (2013) Human tim-tipin complex affects the biochemical properties of the replicative DNA helicase and DNA polymerases. Proc Natl Acad Sci U S A 110 (7): 2523-2527.

NCBI and Uniprot Product Information

• NCBI GI #: 584613506
• NCBI GeneID: 54962
• NCBI Accession #: NP_001276915.1
• NCBI GenBank Nucleotide #: NM_001289986.1
• UniProt Accession #: Q9BVW5; Q9NWZ6; B2CW64
• Molecular Weight: 34,555 Da
• NCBI Official Full Name: TIMELESS-interacting protein isoform 2
• NCBI Official Synonym Full Names: TIMELESS interacting protein
• NCBI Official Symbol: TIPIN
• NCBI Protein Information: TIMELESS-interacting protein; CSM3 homolog
• UniProt Protein Name: TIMELESS-interacting protein
• UniProt Gene Name: TIPIN
• UniProt Entry Name: TIPIN_HUMAN