Standard Curve (Sample)
(Fig.1. Mouse TIMELESS-interacting protein (TIPIN) Standard Curve.)
Mouse TIMELESS-Interacting Protein (TIPIN) ELISA Kit | TIPIN elisa kit
Mouse TIMELESS-Interacting Protein (TIPIN) ELISA Kit
Gene Names
Tipin; 1110005A05Rik; 1110018P21Rik
Reactivity
Mouse
Synonyms
TIMELESS-Interacting Protein (TIPIN); N/A; Mouse TIMELESS-Interacting Protein (TIPIN) ELISA Kit; TIPIN; FLJ20516; timeless-interacting protein; TIPIN elisa kit
Reactivity
Mouse
Specificity
Mouse TIMELESS-interacting protein (TIPIN) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TIPIN. No significant cross-reactivity or interference between Mouse TIPIN and analogues was observed.
Sequence Length
278
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Mouse TIMELESS-interacting protein (TIPIN) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Mouse TIMELESS-interacting protein (TIPIN) Standard Curve.)
Related Product Information for TIPIN elisa kit
Description: This Mouse TIMELESS-interacting protein (TIPIN) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPIN in samples. An antibody specific for TIPIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIPIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPIN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TIPIN associated with the components of the replicative helicase in human cell lines and protected cells against genotoxic agents. TIPIN was required for efficient cell cycle arrest in response to DNA damage, and depletion of TIPIN rendered cells sensitive to ionizing radiation as well as replication stress.It shares 72% amino acid identity with mouse Tipin, with the greatest divergence in the C termini. coexpression of Tim and Tipin in early embryonic and extraembryonic tissues, although Tipin was more widely expressed. After embryonic day 14.5, both Tim and Tipin were abundantly expressed in thymus, liver, gastrointestinal tract, and ventricular zone of brain, and Tipin was also expressed in lung. In adult mouse brain, Tipin was expressed in thalamic and cortical regions, with lower expression in suprachiasmatic nuclei.
Background: TIPIN associated with the components of the replicative helicase in human cell lines and protected cells against genotoxic agents. TIPIN was required for efficient cell cycle arrest in response to DNA damage, and depletion of TIPIN rendered cells sensitive to ionizing radiation as well as replication stress.It shares 72% amino acid identity with mouse Tipin, with the greatest divergence in the C termini. coexpression of Tim and Tipin in early embryonic and extraembryonic tissues, although Tipin was more widely expressed. After embryonic day 14.5, both Tim and Tipin were abundantly expressed in thymus, liver, gastrointestinal tract, and ventricular zone of brain, and Tipin was also expressed in lung. In adult mouse brain, Tipin was expressed in thalamic and cortical regions, with lower expression in suprachiasmatic nuclei.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
TIMELESS-interacting protein
NCBI Official Synonym Full Names
timeless interacting protein
NCBI Official Symbol
Tipin
NCBI Official Synonym Symbols
1110005A05Rik; 1110018P21Rik
NCBI Protein Information
TIMELESS-interacting protein
UniProt Protein Name
TIMELESS-interacting protein
UniProt Gene Name
Tipin
UniProt Entry Name
TIPIN_MOUSE
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Product Notes
The Mouse TIPIN tipin (Catalog #AAA332234) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA332234 ELISA Kit recognizes Mouse TIPIN. It is sometimes possible for the material contained within the vial of "TIMELESS-Interacting Protein (TIPIN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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