IF (Immunofluorescence)
(Staining A549 cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF3648) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Rabbit anti-Human, Rat TIRAP Polyclonal Antibody | anti-TIRAP antibody
Phospho-TIRAP (Tyr106) Antibody
Gene Names
TIRAP; Mal; wyatt; BACTS1; MyD88-2
Reactivity
Human, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
TIRAP, Antibody; Phospho-TIRAP (Tyr106) Antibody; Adapter protein wyatt; FLJ42305; MAL; MyD88 adapter like protein; TIR domain containing adapter protein; Toll interleukin 1 receptor (TIR) domain containing adaptor protein; Toll interleukin 1 receptor domain containing adapter protein; Toll like receptor adaptor protein; Wyatt; anti-TIRAP antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-TIRAP (Tyr106) Antibody detects endogenous levels of TIRAP only when phosphorylated at Tyr106.
Tissue Specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
Tissue Specificity: Highly expressed in liver, kidney, spleen, skeletal muscle and heart. Also detected in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TIRAP antibody
ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
A synthesized peptide derived from human TIRAP around the phosphorylation site of Tyr106.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK.Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation.
Subunit Structure
Homodimer. Also forms heterodimers with MYD88. May interact with PIK3AP1 (By similarity). Interacts with TLR4 and IRAK2 via their respective TIR domains. Interacts with BMX and TBK1. Interacts with EIF2AK2. Does not interact with IRAK1, nor TLR9. (Microbial infection) In case of infection, interacts with Brucella protein BtpA.
Subcellular Location
Cytoplasm. Cell membrane. Membrane.
Note: Colocalizes with DAB2IP at the plasma membrane.
Note: Colocalizes with DAB2IP at the plasma membrane.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(Staining A549 cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF3648) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(Staining A549 cells(heat shock treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AF3648) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
WB (Western Blot)
(Western blot analysis of extracts from Rat heart, using Phospho-TIRAP (Tyr106) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Rat heart, using Phospho-TIRAP (Tyr106) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-TIRAP antibody
Adapter involved in TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response. Positively regulates the production of TNF-alpha and interleukin-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,883 Da
NCBI Official Full Name
toll/interleukin-1 receptor domain-containing adapter protein isoform a
NCBI Official Synonym Full Names
toll-interleukin 1 receptor (TIR) domain containing adaptor protein
NCBI Official Symbol
TIRAP
NCBI Official Synonym Symbols
Mal; wyatt; BACTS1; MyD88-2
NCBI Protein Information
toll/interleukin-1 receptor domain-containing adapter protein; adapter protein wyatt; adaptor protein Wyatt; MyD88 adapter-like protein; Toll-like receptor adaptor protein
UniProt Protein Name
Toll/interleukin-1 receptor domain-containing adapter protein
UniProt Gene Name
TIRAP
UniProt Synonym Gene Names
MAL; TIR domain-containing adapter protein; MyD88-2
UniProt Entry Name
TIRAP_HUMAN
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Product Notes
The TIRAP tirap (Catalog #AAA329267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-TIRAP (Tyr106) Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TIRAP can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the TIRAP tirap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIRAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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