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IHC (Immunohistochemistry)
(TIRC7/TCIRG1 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit anti-Mouse, Human TIRC7/TCIRG1 Polyclonal Antibody | anti-TCIRG1 antibody
TIRC7/TCIRG1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Gene Names
TCIRG1; a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa
Reactivity
Mouse, Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein A and Peptide Affinity Chromatography
Synonyms
TIRC7/TCIRG1, Antibody; TIRC7/TCIRG1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody; IHC-plus TIRC7/TCIRG1 Antibody (C-Terminus); TCIRG1; A3; ATP6N1C; ATP6V0A3; ATP6N1D; Atp6i; OPTB1; OC-116; STV1; T-cell immune response cDNA 7; V- ATPase 116-kDa isoform a3; V-ATPase 116 kDa isoform a3; TIRC7; OC-116kDa; OC116; T-cell immune regulator 1; ATPase; H+ transporting; 116kD; V-ATPase 116-kDa; OC-116 kDa; T-cell; immune regulator 1; VPH1; anti-TCIRG1 antibody
Host
Rabbit
Reactivity
Mouse, Human
Clonality
Polyclonal
Isotype
Ig
Purity/Purification
Protein A and Peptide Affinity Chromatography
Form/Format
PBS, 0.09% Sodium Azide
Applicable Applications for anti-TCIRG1 antibody
WB (Western Blot), ELISA (Peptide ELISA), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Target
Human TIRC7/TCIRG1
Immunogen
This TCIRG1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 668-702 amino acids from the human region of human TCIRG1.
Conjugation
Unconjugated
Epitope
C-Terminus
Family
Transporter
Subfamily
ATPase-V type
Preparation and Storage
Short Term: Store at 2-8 degree C for up to 2 weeks. Long Term: Aliquot and store at -20 degree C. Avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(TIRC7/TCIRG1 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(TIRC7/TCIRG1 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(TIRC7/TCIRG1 Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(TIRC7/TCIRG1 Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(TIRC7/TCIRG1 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemisry)
(TIRC7/TCIRG1 Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(TIRC7/TCIRG1 Antibody-Anti-TCIRG1 Antibody (C-Term) at 1:2000 dilution + EL4 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(TIRC7/TCIRG1 Antibody-Anti-TCIRG1 Antibody (C-Term) at 1:2000 dilution + EL4 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
FCM/FACS (Flow Cytometry)
(TIRC7/TCIRG1 Antibody-Overlay histogram showing U-2 OS cells stained with TCIRG1 Antibody (C-Term)(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (TCIRG1 Antibody (C-Term), 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
FCM/FACS (Flow Cytometry)
(TIRC7/TCIRG1 Antibody-Overlay histogram showing U-2 OS cells stained with TCIRG1 Antibody (C-Term)(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (TCIRG1 Antibody (C-Term), 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Related Product Information for anti-TCIRG1 antibody
TCIRG1 antibody is an unconjugated rabbit polyclonal antibody to TCIRG1 (TIRC7)(C-Terminus) from human. It is reactive with human and mouse. Validated for Flow, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,776 Da
NCBI Official Full Name
V-type proton ATPase 116 kDa subunit a isoform 3 isoform a
NCBI Official Synonym Full Names
T-cell immune regulator 1, ATPase H+ transporting V0 subunit a3
NCBI Official Symbol
TCIRG1
NCBI Official Synonym Symbols
a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa
NCBI Protein Information
V-type proton ATPase 116 kDa subunit a isoform 3
UniProt Protein Name
V-type proton ATPase 116 kDa subunit a isoform 3
UniProt Gene Name
TCIRG1
UniProt Synonym Gene Names
ATP6N1C; ATP6V0A3; V-ATPase 116 kDa isoform a3; OC-116 kDa; OC116; TIRC7
UniProt Entry Name
VPP3_HUMAN
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Product Notes
The TCIRG1 tcirg1 (Catalog #AAA163344) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIRC7/TCIRG1 Rabbit anti-Human Polyclonal (C-Terminus) Antibody reacts with Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIRC7/TCIRG1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA (Peptide ELISA), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TCIRG1 tcirg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIRC7/TCIRG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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