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IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100.)
Rabbit anti-Human, Mouse TLR4 Polyclonal Antibody | anti-TLR4 antibody
TLR4 Antibody
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
TLR4, Antibody; TLR4 Antibody; ARMD10; CD284; TLR 4; TOLL; Toll like receptor 4; anti-TLR4 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Form/Format
Affinity Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
839
Applicable Applications for anti-TLR4 antibody
WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Certificate of Analysis
2 ug/mL of SPC-200 was sufficient for detection of TLR4 in 100ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Conjugate
Unconjugated
Storage Buffer
PBS, 0.05% BSA, 0.05% sodium azide
Preparation and Storage
-20 degree C
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: Spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100.)
IHC (Immunohistochemisry)
(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: Spleen tissue. Species: Mouse. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100.)
WB (Western Blot)
(Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:1000.)
WB (Western Blot)
(Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:1000.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells.)
Related Product Information for anti-TLR4 antibody
Background Info: Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
Scientific Background: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).
Scientific Background: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90kDa (6).
Product Categories/Family for anti-TLR4 antibody
References
1.JMuzio M., Natoli G., Saccan S., Levrero M., and Mantovani A. (1998) J. Exp. Med. 187: 2097-2101. 2. Medzhitov R. and Janeway C.A (1997). Cell 91: 295-298. 3. Chuang T.H. and Ulevitch R.J. (2001) Biochim. Biophys. Acta 1518 (1-2): 157-161). 4. Takeuchi O., et al. (1999) Immunity 11: 443. 5. Poltorak A., Riccardi-Castagnoli P., Citterio S., and Butler B. (2000) Proc. Natl. Acad. Sci USA 97: 2163-2167. 6. Medzhitov R., Preston-Hurlburt P. and Janeway C.A. (1997) Jr. Nature 388 (6640): 394-397.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
73,301 Da
NCBI Official Full Name
toll-like receptor 4 isoform A
NCBI Official Synonym Full Names
toll-like receptor 4
NCBI Official Symbol
TLR4
NCBI Official Synonym Symbols
TOLL; CD284; TLR-4; ARMD10
NCBI Protein Information
toll-like receptor 4; hToll; homolog of Drosophila toll
UniProt Protein Name
Toll-like receptor 4
UniProt Gene Name
TLR4
UniProt Entry Name
TLR4_HUMAN
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Product Notes
The TLR4 tlr4 (Catalog #AAA103610) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TLR4 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TLR4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the TLR4 tlr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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