WB (Western Blot)
(Western blot analysis of extracts of Mouse Liver tissue sample,using Phospho-TLR5 (Ser805) Antibody.)
Rabbit TLR5 Polyclonal Antibody | anti-TLR5 antibody
Phospho-TLR5 (Ser805) Antibody
Gene Names
TLR5; SLE1; TIL3; SLEB1; MELIOS
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Sheep, Rabbit
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
TLR5, Antibody; Phospho-TLR5 (Ser805) Antibody; FLJ10052; MGC126430; MGC126431; SLEB1; TIL 3; TIL3; TLR 5; Tlr5; TLR5_HUMAN; Toll like receptor 5; Toll like receptor 5 precursor; Toll-like receptor 5; Toll/interleukin 1 receptor like protein 3; Toll/interleukin-1 receptor-like protein 3; anti-TLR5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Sheep, Rabbit
Predicted: Pig, Bovine, Sheep, Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-TLR5 (Ser805) Antibody detects endogenous levels of TLR5 only when phosphorylated at Ser805.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
858
Applicable Applications for anti-TLR5 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human TLR5 around the phosphorylation site of Ser805.
Tissue Specificity
Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.
Subcellular Location
Membrane
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts of Mouse Liver tissue sample,using Phospho-TLR5 (Ser805) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts of Mouse Liver tissue sample,using Phospho-TLR5 (Ser805) Antibody.)
IHC (Immunohiostchemistry)
(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-TLR5 antibody
Description: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Post Translational Modifications: Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines. Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
Subunit Structure: Binds MYD88 via their respective TIR domains (By similarity). Homodimer both in the absence and presence of ligand.
Similarity: Belongs to the Toll-like receptor family.
Post Translational Modifications: Phosphorylated at Ser-805 by PKD/PRKD1; phosphorylation induces the production of inflammatory cytokines. Phosphorylated at Tyr-798 upon flagellin binding; required for signaling.
Subunit Structure: Binds MYD88 via their respective TIR domains (By similarity). Homodimer both in the absence and presence of ligand.
Similarity: Belongs to the Toll-like receptor family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 98 kDa
Predicted: 98 kDa
Predicted: 98 kDa
NCBI Official Full Name
Toll-like receptor 5
NCBI Official Synonym Full Names
toll like receptor 5
NCBI Official Symbol
TLR5
NCBI Official Synonym Symbols
SLE1; TIL3; SLEB1; MELIOS
NCBI Protein Information
toll-like receptor 5
UniProt Protein Name
Toll-like receptor 5
UniProt Gene Name
TLR5
UniProt Synonym Gene Names
TIL3
UniProt Entry Name
TLR5_HUMAN
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Product Notes
The TLR5 tlr5 (Catalog #AAA328309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-TLR5 (Ser805) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Sheep, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's TLR5 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TLR5 tlr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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